Recently, the tissue origin of MDA-MB-435 cell line has been the subject of considerable debate. In this study, we set out to determine whether MDA-MB-435-DTP cells shown to express melanoma-specific genes were identical to various other MDA-MB-435 cell stocks worldwide. CGH-microarray, genetic polymorphism genotyping, microsatellite fingerprint analysis and/or chromosomal number confirmed that the MDA-MB-435 cells maintained at the Lombardi Comprehensive Cancer Center (MDA-MB-435-LCC) are almost identical to the MDA-MB-435-DTP cells, and showed a very similar profile to those obtained from the same original source (MD Anderson Cancer Center) but maintained independently (MDA-MB-435-PMCC). Gene expression profile analy-sis confirmed common expression of genes among different MDA-MB-435-LCC cell stocks, and identified some unique gene products in MDA-MB-435-PMCC cells. RT-PCR analysis confirmed the expression of the melanoma marker tyrosinase across multiple MDA-MB-435 cell stocks. Collectively, our results show that the MDA-MB-435 cells used widely have identical origins to those that exhibit a melanoma-like gene expression signature, but exhibit a small degree of genotypic and phenotypic drift.
Bibliographical noteFunding Information:
The authors acknowledge the collegiality of Dr. Janet Price, MD Anderson Cancer Center for comments and assistance with this manuscript. Fleur Hammet, Melanie de Silva, Anne-Marie Hutchins from the Molecular Pathology laboratory of the Victorian Breast Cancer Research Consortium are thanked for their assistance. The authors also wish to thank Dr Dominic A. Scu-diero of the National Institutes of Health for DNA and RNA from MDA-MB-435-DTP cells. This work was primarily supported by the Victorian Breast Cancer Research Consortium and grants from the Breast Cancer Research Foundation (N003173), US-DOD (BC 021320) and NIH (R21 CA87244-01). We thank the Microscopy and Imaging, Tissue Culture, Biostatistics, Research Computing, and Macromolecular Shared Resources of the Lombardi Cancer Center, which are partially supported by PHS grant NIH 1P30-CA-51008 (Cancer Center Support Grant) to Lombardi Cancer Center.
- Breast cancer
- Cell lines
- Chromosomal number
- Microsatellite analysis