Commentary on human pluripotent stem cell-based blood–brain barrier models

Ethan S. Lippmann, Samira M. Azarin, Sean P. Palecek, Eric V. Shusta

Research output: Contribution to journalComment/debatepeer-review


In 2012, we provided the first published evidence that human pluripotent stem cells could be differentiated to cells exhibiting markers and phenotypes characteristic of the blood–brain barrier (BBB). In the ensuing years, the initial protocols have been refined, and the research community has identified both positive and negative attributes of this stem cell-based BBB model system. Here, we give our perspective on the current status of these models and their use in the BBB community, as well as highlight key attributes that would benefit from improvement moving forward.

Original languageEnglish (US)
Article number64
JournalFluids and Barriers of the CNS
Issue number1
StatePublished - Dec 1 2020

Bibliographical note

Funding Information:
This work was funded in part by grants from the National Institutes of Health (NS103844 and NS107461 to EVS and SPP). Acknowledgements


  • Blood–brain barrier
  • Human pluripotent stem cell
  • In vitro model

PubMed: MeSH publication types

  • Editorial

Fingerprint Dive into the research topics of 'Commentary on human pluripotent stem cell-based blood–brain barrier models'. Together they form a unique fingerprint.

Cite this