In 2012, we provided the first published evidence that human pluripotent stem cells could be differentiated to cells exhibiting markers and phenotypes characteristic of the blood–brain barrier (BBB). In the ensuing years, the initial protocols have been refined, and the research community has identified both positive and negative attributes of this stem cell-based BBB model system. Here, we give our perspective on the current status of these models and their use in the BBB community, as well as highlight key attributes that would benefit from improvement moving forward.
Bibliographical noteFunding Information:
This work was funded in part by grants from the National Institutes of Health (NS103844 and NS107461 to EVS and SPP). Acknowledgements
- Blood–brain barrier
- Human pluripotent stem cell
- In vitro model
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