Combining multiple nutrient stresses and bicarbonate addition to promote lipid accumulation in the diatom RGd-1

K. M. Moll; Rob Gardner; E. O. Eustance; R. Gerlach; B. M. Peyton

doi:10.1016/j.algal.2014.04.002

Combining multiple nutrient stresses and bicarbonate addition to promote lipid accumulation in the diatom RGd-1

K. M. Moll, Rob Gardner, E. O. Eustance, R. Gerlach, B. M. Peyton

West Central Research and Outreach Center

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Algal biofuels represent a renewable, potentially viable, solution to mitigate transportation fuel demands. A novel diatom strain, RGd-1, isolated from Yellowstone National Park, produces high concentrations of lipids that can be converted to biodiesel. To increase the cell concentration and determine optimal conditions for growth, RGd-1 was grown without added Si, in the presence of four Si concentrations within the soluble range (0.5-2mM), and one above the soluble range (2.5mM). Medium Si concentrations and intracellular triacylglycerol (TAG) content were monitored daily by inductively coupled plasma mass spectrometry and Nile Red fluorescence, respectively (end-point TAG values were measured using gas chromatography). Si depletion with or without combined nitrate (NO₃^-) limitation was shown to induce TAG accumulation. Additionally, the effects of sodium bicarbonate (NaHCO₃) supplementation were examined on cultures grown using two NO₃^- concentrations (2.94 and 1mM NO₃^-), which also resulted in increased TAG accumulation. It was determined that utilizing a combination of two independent physiological stresses and HCO₃^- supplementation resulted in the highest total and per cell TAG accumulation.

Original language	English (US)
Pages (from-to)	7-15
Number of pages	9
Journal	Algal Research
Volume	5
Issue number	1
DOIs	https://doi.org/10.1016/j.algal.2014.04.002
State	Published - 2014

Bibliographical note

Funding Information:
The authors would like to thank all members of the MSU Algal Biofuels Group. Additionally, the authors especially thank John Barnick and Ellen Lauchnor for their help with running the ICP-MS, Ann Willis at the CBE for assistance in running IC, as well as Hannah Newhouse and Dan McDonald for assisting with clone libraries and Nate Murphy for experimental support. The authors acknowledge Yellowstone National Park as well as funding for the establishment and operation of the Environmental and Biofilm Mass Spectrometry Facility at Montana State University (MSU) through the Defense University Research Instrumentation Program (DURIP, Contract Number: W911NF0510255) and the MSU Thermal Biology Institute from the NASA Exobiology Program (Project NAG5-8807). Funding Sources: U.S. Department of Energy : Office of Biomass Programs grant DE-FG36-08G018161 , Office of Energy Efficiency and Renewable Energy (EERE) Biomass Program under Contract No. DE-EE0003136 and National Science Foundation CHE-1230632 . This work was additionally made possible by Betsy Pitts and the microscopy facilities at the Center for Biofilm Engineering, which was supported by funding obtained from the NSF-MRI Program and the M.J. Murdock Charitable Trust .

Publisher Copyright:
© 2014 Elsevier B.V.

Keywords

Algae
Biodiesel
Diatoms
Lipids
Silica
Sodium bicarbonate

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Combining multiple nutrient stresses and bicarbonate addition to promote lipid accumulation in the diatom RGd-1",

abstract = "Algal biofuels represent a renewable, potentially viable, solution to mitigate transportation fuel demands. A novel diatom strain, RGd-1, isolated from Yellowstone National Park, produces high concentrations of lipids that can be converted to biodiesel. To increase the cell concentration and determine optimal conditions for growth, RGd-1 was grown without added Si, in the presence of four Si concentrations within the soluble range (0.5-2mM), and one above the soluble range (2.5mM). Medium Si concentrations and intracellular triacylglycerol (TAG) content were monitored daily by inductively coupled plasma mass spectrometry and Nile Red fluorescence, respectively (end-point TAG values were measured using gas chromatography). Si depletion with or without combined nitrate (NO3-) limitation was shown to induce TAG accumulation. Additionally, the effects of sodium bicarbonate (NaHCO3) supplementation were examined on cultures grown using two NO3- concentrations (2.94 and 1mM NO3-), which also resulted in increased TAG accumulation. It was determined that utilizing a combination of two independent physiological stresses and HCO3- supplementation resulted in the highest total and per cell TAG accumulation.",

keywords = "Algae, Biodiesel, Diatoms, Lipids, Silica, Sodium bicarbonate",

author = "Moll, {K. M.} and Rob Gardner and Eustance, {E. O.} and R. Gerlach and Peyton, {B. M.}",

note = "Funding Information: The authors would like to thank all members of the MSU Algal Biofuels Group. Additionally, the authors especially thank John Barnick and Ellen Lauchnor for their help with running the ICP-MS, Ann Willis at the CBE for assistance in running IC, as well as Hannah Newhouse and Dan McDonald for assisting with clone libraries and Nate Murphy for experimental support. The authors acknowledge Yellowstone National Park as well as funding for the establishment and operation of the Environmental and Biofilm Mass Spectrometry Facility at Montana State University (MSU) through the Defense University Research Instrumentation Program (DURIP, Contract Number: W911NF0510255) and the MSU Thermal Biology Institute from the NASA Exobiology Program (Project NAG5-8807). Funding Sources: U.S. Department of Energy : Office of Biomass Programs grant DE-FG36-08G018161 , Office of Energy Efficiency and Renewable Energy (EERE) Biomass Program under Contract No. DE-EE0003136 and National Science Foundation CHE-1230632 . This work was additionally made possible by Betsy Pitts and the microscopy facilities at the Center for Biofilm Engineering, which was supported by funding obtained from the NSF-MRI Program and the M.J. Murdock Charitable Trust . Publisher Copyright: {\textcopyright} 2014 Elsevier B.V.",

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doi = "10.1016/j.algal.2014.04.002",

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AU - Moll, K. M.

AU - Gardner, Rob

AU - Eustance, E. O.

AU - Gerlach, R.

AU - Peyton, B. M.

N1 - Funding Information: The authors would like to thank all members of the MSU Algal Biofuels Group. Additionally, the authors especially thank John Barnick and Ellen Lauchnor for their help with running the ICP-MS, Ann Willis at the CBE for assistance in running IC, as well as Hannah Newhouse and Dan McDonald for assisting with clone libraries and Nate Murphy for experimental support. The authors acknowledge Yellowstone National Park as well as funding for the establishment and operation of the Environmental and Biofilm Mass Spectrometry Facility at Montana State University (MSU) through the Defense University Research Instrumentation Program (DURIP, Contract Number: W911NF0510255) and the MSU Thermal Biology Institute from the NASA Exobiology Program (Project NAG5-8807). Funding Sources: U.S. Department of Energy : Office of Biomass Programs grant DE-FG36-08G018161 , Office of Energy Efficiency and Renewable Energy (EERE) Biomass Program under Contract No. DE-EE0003136 and National Science Foundation CHE-1230632 . This work was additionally made possible by Betsy Pitts and the microscopy facilities at the Center for Biofilm Engineering, which was supported by funding obtained from the NSF-MRI Program and the M.J. Murdock Charitable Trust . Publisher Copyright: © 2014 Elsevier B.V.

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N2 - Algal biofuels represent a renewable, potentially viable, solution to mitigate transportation fuel demands. A novel diatom strain, RGd-1, isolated from Yellowstone National Park, produces high concentrations of lipids that can be converted to biodiesel. To increase the cell concentration and determine optimal conditions for growth, RGd-1 was grown without added Si, in the presence of four Si concentrations within the soluble range (0.5-2mM), and one above the soluble range (2.5mM). Medium Si concentrations and intracellular triacylglycerol (TAG) content were monitored daily by inductively coupled plasma mass spectrometry and Nile Red fluorescence, respectively (end-point TAG values were measured using gas chromatography). Si depletion with or without combined nitrate (NO3-) limitation was shown to induce TAG accumulation. Additionally, the effects of sodium bicarbonate (NaHCO3) supplementation were examined on cultures grown using two NO3- concentrations (2.94 and 1mM NO3-), which also resulted in increased TAG accumulation. It was determined that utilizing a combination of two independent physiological stresses and HCO3- supplementation resulted in the highest total and per cell TAG accumulation.

AB - Algal biofuels represent a renewable, potentially viable, solution to mitigate transportation fuel demands. A novel diatom strain, RGd-1, isolated from Yellowstone National Park, produces high concentrations of lipids that can be converted to biodiesel. To increase the cell concentration and determine optimal conditions for growth, RGd-1 was grown without added Si, in the presence of four Si concentrations within the soluble range (0.5-2mM), and one above the soluble range (2.5mM). Medium Si concentrations and intracellular triacylglycerol (TAG) content were monitored daily by inductively coupled plasma mass spectrometry and Nile Red fluorescence, respectively (end-point TAG values were measured using gas chromatography). Si depletion with or without combined nitrate (NO3-) limitation was shown to induce TAG accumulation. Additionally, the effects of sodium bicarbonate (NaHCO3) supplementation were examined on cultures grown using two NO3- concentrations (2.94 and 1mM NO3-), which also resulted in increased TAG accumulation. It was determined that utilizing a combination of two independent physiological stresses and HCO3- supplementation resulted in the highest total and per cell TAG accumulation.

KW - Algae

KW - Biodiesel

KW - Diatoms

KW - Lipids

KW - Silica

KW - Sodium bicarbonate

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VL - 5

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JO - Algal Research

JF - Algal Research

IS - 1

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Combining multiple nutrient stresses and bicarbonate addition to promote lipid accumulation in the diatom RGd-1

Abstract

Bibliographical note

Keywords

UN SDGs

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