Combined Mössbauer and EPR Studies of the S = 3 State of an Exchange-Coupled FeIIICuII Complex: Test for Quantitative EPR Analysis of Integer Spin Systems

C. Juarez-Garcia, M. P. Hendrich, T. R. Holman, L. Que, E. Münck

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Abstract

We have studied acetonitrile solutions of the bimetallic complex [FeIIICuII(BPMP)Cl2](BPh4)2, where BPMP is the anion 2,6-bis[(bis(2-pyridylmethyl)amino)methyl]-4-methylphenol, with Mössbauer and EPR spectroscopy. Both spectroscopic techniques show that the complex is ferromagnetically coupled (Ĥ = JS1·S2 J < 0) to yield a ground state spin S = 3. Analysis of the Mössbauer spectra taken in applied fields up to 6.0 T yielded for the FeIII site the zero-field splitting parameters D1 = +1.2 cm−1 and E1 = 0.11 cm−1, the magnetic hyperfine coupling constant A0 = −28.8 MHz, quadrupole spitting ΔEQ = 0.67 mm/s, and isomer shift δ = 0.48 mm/s. The zero-field splitting term of the ferric ion mixes the excited S = 2 multiplet with the ground manifold, the mixing being proportional to D1/J; by analyzing this mixing we have determined that −2 cm−1 > J > −5 cm−1. The FeIIICuII complex gives an X-band resonance at g = 12 which we have studied with a bimodal cavity in the temperature range from 2 to 50 K. We have analyzed the position and line shape of the g = 12 signal with a computer program in the framework of an S = 3 spin Hamiltonian, allowing a Gaussian distribution of the zero-field splitting parameters D and E. Finally, we have determined the S = 3 spin concentration from spectral simulations relative to a known standard; the value obtained agrees with the optically determined concentration. Our result shows that it is possible to determine spin concentrations of integer spin complexes with a precision that approaches that obtained for systems with half-integral spin. The availability of such techniques is highly desirable for the study of an ever increasing number of metalloproteins. We describe a method that allows one to determine the spin of the coupled system directly from the Mössbauer spectrum. Using this method we show here that the ground manifolds of [FeIIICuII(BPMP)Cl2](BPh4)2 and [FeIII,CuIIBPMP(OAc)2](BPh4)2 have S = 3 and 2, respectively. At T = 93 K the zero-field Mössbauer spectrum of the integer spin complex [Fe1IICu“(BPMP)Cl2](BPh4)2 exhibits unusual line broadening, similar to that observed for Thermus thermophilus cytochrome c1aa3.

Original languageEnglish (US)
Pages (from-to)518-525
Number of pages8
JournalJournal of the American Chemical Society
Volume113
Issue number2
DOIs
StatePublished - Jan 1 1991

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