Combined chromosome microdissection and comparative genomic hybridization detect multiple sites of amplified DNA in a human lung carcinoma cell line

Takahiro Taguchi; George Z. Cheng; Daphne W. Bell; Binaifer Balsara; Zemin Liu; Jill M. Siegfried; Joseph R. Testa

doi:10.1002/(SICI)1098-2264(199710)20:2<208::AID-GCC13>3.0.CO;2-M

Combined chromosome microdissection and comparative genomic hybridization detect multiple sites of amplified DNA in a human lung carcinoma cell line

Takahiro Taguchi
, George Z. Cheng
, Daphne W. Bell
, Binaifer Balsara
, Zemin Liu
, Jill M. Siegfried
, Joseph R. Testa

Pharmacology

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Chromosome microdissection-fluorescence in situ hybridization and comparative genomic hybridization (CGH) were performed in parallel to identify the native location of amplified DNA in a human non-small cell lung cancer (NSCLC) cell line exhibiting a homogeneously staining region (hsr) and double minutes (dmin). The native locations of microdissected DNA from the hsr and drain were 7p12-13 and 8q24, respectively. Southern analysis revealed coamplification of EGFR (7p12) and MYC (8q24). CGH detected amplification of DNA not only from 7p12-13 and 8q24, but also from 9p24 and 10q22.

Original language	English (US)
Pages (from-to)	208-212
Number of pages	5
Journal	Genes Chromosomes and Cancer
Volume	20
Issue number	2
DOIs	https://doi.org/10.1002/(SICI)1098-2264(199710)20:2<208::AID-GCC13>3.0.CO;2-M
State	Published - Oct 1997

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Taguchi, T., Cheng, G. Z., Bell, D. W., Balsara, B., Liu, Z., Siegfried, J. M., & Testa, J. R. (1997). Combined chromosome microdissection and comparative genomic hybridization detect multiple sites of amplified DNA in a human lung carcinoma cell line. Genes Chromosomes and Cancer, 20(2), 208-212. https://doi.org/10.1002/(SICI)1098-2264(199710)20:2<208::AID-GCC13>3.0.CO;2-M

Taguchi, T, Cheng, GZ, Bell, DW, Balsara, B, Liu, Z, Siegfried, JM & Testa, JR 1997, 'Combined chromosome microdissection and comparative genomic hybridization detect multiple sites of amplified DNA in a human lung carcinoma cell line', Genes Chromosomes and Cancer, vol. 20, no. 2, pp. 208-212. https://doi.org/10.1002/(SICI)1098-2264(199710)20:2<208::AID-GCC13>3.0.CO;2-M

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abstract = "Chromosome microdissection-fluorescence in situ hybridization and comparative genomic hybridization (CGH) were performed in parallel to identify the native location of amplified DNA in a human non-small cell lung cancer (NSCLC) cell line exhibiting a homogeneously staining region (hsr) and double minutes (dmin). The native locations of microdissected DNA from the hsr and drain were 7p12-13 and 8q24, respectively. Southern analysis revealed coamplification of EGFR (7p12) and MYC (8q24). CGH detected amplification of DNA not only from 7p12-13 and 8q24, but also from 9p24 and 10q22.",

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doi = "10.1002/(SICI)1098-2264(199710)20:2<208::AID-GCC13>3.0.CO;2-M",

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AU - Taguchi, Takahiro

AU - Cheng, George Z.

AU - Bell, Daphne W.

AU - Balsara, Binaifer

AU - Liu, Zemin

AU - Siegfried, Jill M.

AU - Testa, Joseph R.

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AB - Chromosome microdissection-fluorescence in situ hybridization and comparative genomic hybridization (CGH) were performed in parallel to identify the native location of amplified DNA in a human non-small cell lung cancer (NSCLC) cell line exhibiting a homogeneously staining region (hsr) and double minutes (dmin). The native locations of microdissected DNA from the hsr and drain were 7p12-13 and 8q24, respectively. Southern analysis revealed coamplification of EGFR (7p12) and MYC (8q24). CGH detected amplification of DNA not only from 7p12-13 and 8q24, but also from 9p24 and 10q22.

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Combined chromosome microdissection and comparative genomic hybridization detect multiple sites of amplified DNA in a human lung carcinoma cell line

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