Combined chromosome microdissection and comparative genomic hybridization detect multiple sites of amplified DNA in a human lung carcinoma cell line

Takahiro Taguchi, George Z. Cheng, Daphne W. Bell, Binaifer Balsara, Zemin Liu, Jill M. Siegfried, Joseph R. Testa

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Chromosome microdissection-fluorescence in situ hybridization and comparative genomic hybridization (CGH) were performed in parallel to identify the native location of amplified DNA in a human non-small cell lung cancer (NSCLC) cell line exhibiting a homogeneously staining region (hsr) and double minutes (dmin). The native locations of microdissected DNA from the hsr and drain were 7p12-13 and 8q24, respectively. Southern analysis revealed coamplification of EGFR (7p12) and MYC (8q24). CGH detected amplification of DNA not only from 7p12-13 and 8q24, but also from 9p24 and 10q22.

Original languageEnglish (US)
Pages (from-to)208-212
Number of pages5
JournalGenes Chromosomes and Cancer
Volume20
Issue number2
DOIs
StatePublished - Oct 1997

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