Abstract
Chromosome microdissection-fluorescence in situ hybridization and comparative genomic hybridization (CGH) were performed in parallel to identify the native location of amplified DNA in a human non-small cell lung cancer (NSCLC) cell line exhibiting a homogeneously staining region (hsr) and double minutes (dmin). The native locations of microdissected DNA from the hsr and drain were 7p12-13 and 8q24, respectively. Southern analysis revealed coamplification of EGFR (7p12) and MYC (8q24). CGH detected amplification of DNA not only from 7p12-13 and 8q24, but also from 9p24 and 10q22.
Original language | English (US) |
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Pages (from-to) | 208-212 |
Number of pages | 5 |
Journal | Genes Chromosomes and Cancer |
Volume | 20 |
Issue number | 2 |
DOIs | |
State | Published - Oct 1997 |