A liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI+-MS/MS) method for the analysis of the tobacco-specific carcinogens N'-nitrosonornicotine (NNN) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) and their glucuronides (total NNN and total NNAL) in human urine was developed. The method has excellent accuracy and intra-day and inter-day precision, and limits of quantitation of 0.015 and 0.075pmol/mL urine, respectively, for total NNN and total NNAL. A unique aspect of this method is internal assessment of possible artifactual formation of NNN by inclusion of the monitor amine [pyridine-D4]nornicotine. We found that artifactual formation of NNN comprised only 2.5% of the measured amounts of total NNN in urine of cigarette smokers, under our conditions using ammonium sulfamate as an inhibitor of nitrosation. The method was applied to urine samples from cigarette smokers and e-cigarette users. Levels of total NNN and total NNAL in the urine of cigarette smokers averaged 0.060±0.035pmol/mL and 2.41±1.41pmol/mL urine, (N=38), respectively, which were both significantly greater than in the urine of 27 e-cigarette users.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences|
|State||Published - Dec 15 2015|
Bibliographical noteFunding Information:
This study was supported by the U.S. National Institutes of Health CA-81301 , U54 DA-031659 , and U19CA-157345 . Mass spectrometry was carried out in the Analytical Biochemistry Shared Resource of the Masonic Cancer Center, University of Minnesota, funded in part by Cancer Center Support Grant CA-77598 . We thank Peter Villalta and Xun Ming for their valuable help with mass spectrometry. Nornicotine data were kindly provided by the laboratory of Professor S.E. Murphy. We thank Bob Carlson for editorial assistance.
© 2015 Elsevier B.V.
Copyright 2015 Elsevier B.V., All rights reserved.
- Combined analysis
- E-cigarette users' urine
- Liquid chromatography-electrospray ionization-tandem mass spectrometry
- Smokers' urine