Combinatorial control of messenger RNAs by Pumilio, Nanos and Brain Tumor Proteins

René M. Arvola, Chase A. Weidmann, Traci M. Tanaka Hall, Aaron Goldstrohm

Research output: Contribution to journalReview article

18 Citations (Scopus)

Abstract

Eukaryotes possess a vast array of RNA-binding proteins (RBPs) that affect mRNAs in diverse ways to control protein expression. Combinatorial regulation of mRNAs by RBPs is emerging as the rule. No example illustrates this as vividly as the partnership of 3 Drosophila RBPs, Pumilio, Nanos and Brain Tumor, which have overlapping functions in development, stem cell maintenance and differentiation, fertility and neurologic processes. Here we synthesize 30 y of research with new insights into their molecular functions and mechanisms of action. First, we provide an overview of the key properties of each RBP. Next, we present a detailed analysis of their collaborative regulatory mechanism using a classic example of the developmental morphogen, hunchback, which is spatially and temporally regulated by the trio during embryogenesis. New biochemical, structural and functional analyses provide insights into RNA recognition, cooperativity, and regulatory mechanisms. We integrate these data into a model of combinatorial RNA binding and regulation of translation and mRNA decay. We then use this information, transcriptome wide analyses and bioinformatics predictions to assess the global impact of Pumilio, Nanos and Brain Tumor on gene regulation. Together, the results support pervasive, dynamic post-transcriptional control.

Original languageEnglish (US)
Pages (from-to)1445-1456
Number of pages12
JournalRNA Biology
Volume14
Issue number11
DOIs
StatePublished - Nov 2 2017

Fingerprint

RNA-Binding Proteins
Brain Neoplasms
Messenger RNA
Proteins
RNA
Drosophila Proteins
RNA Stability
Gene Expression Profiling
Computational Biology
Eukaryota
Nervous System
Embryonic Development
Fertility
Cell Differentiation
Stem Cells
Maintenance
Research
Genes

Keywords

  • Brain Tumor (or Brat)
  • Nanos
  • Pumilio
  • combinatorial mRNA regulation
  • cooperative RNA binding

Cite this

Combinatorial control of messenger RNAs by Pumilio, Nanos and Brain Tumor Proteins. / Arvola, René M.; Weidmann, Chase A.; Tanaka Hall, Traci M.; Goldstrohm, Aaron.

In: RNA Biology, Vol. 14, No. 11, 02.11.2017, p. 1445-1456.

Research output: Contribution to journalReview article

Arvola, René M. ; Weidmann, Chase A. ; Tanaka Hall, Traci M. ; Goldstrohm, Aaron. / Combinatorial control of messenger RNAs by Pumilio, Nanos and Brain Tumor Proteins. In: RNA Biology. 2017 ; Vol. 14, No. 11. pp. 1445-1456.
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