Combination of micellar electrokinetic and high-performance liquid chromatographies to assess age-related changes in the in vitro metabolism of fischer 344 rat liver

Yaohua Wang, Joseph B. Katzenmeyer, Edgar A. Arriaga

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The metabolism of doxorubicin, a widely used anticancer drug, is different in young adult and old cancer patients. In this study, we demonstrate that micellar electrokinetic chromatography with laser-induced fluorescence detection is highly suited to monitor the metabolism of doxorubicin in subcellular fractions isolated from young adult (11 months, 100% survival rate) and old (26 months, ~25% survival rate) Fischer 344 rat livers. The relative amounts of doxorubicin metabolized in both mitochondria-enriched and postmitochondria fractions of young adult were larger than the respective fractions of old rat liver. 7-Deoxydoxorubicinolone and 7-deoxydoxorubicinone were identified using internal standard addition and structural elucidation by high-performance liquid chromatography with combined laser-induced fluorescence and mass spectrometry detection. Although high-performance liquid chromatography with combined laser-induced fluorescence and mass spectrometry detection is more useful in the identification of compounds, micellar electrokinetic chromatography with laser-induced fluorescence detection has low-sample requirements, simplified sample processing procedures, short analysis times and low limit of detection. Therefore, the combination of these two techniques provides a powerful approach to investigate metabolism of fluorescent drugs in aging studies.

Original languageEnglish (US)
Pages (from-to)935-943
Number of pages9
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume66 A
Issue number9
DOIs
StatePublished - Sep 1 2011

Keywords

  • Capillary electrophoresis
  • Doxorubicin
  • Fluorescence
  • HPLC
  • MS
  • Metabolism

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