Combination of hydrogen fermentation and methanogenesis to enhance energy conversion efficiency from trehalose

Ao Xia, Jun Cheng, Richen Lin, Lingkan Ding, Junhu Zhou, Kefa Cen

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Trehalose was pretreated by microwave heating with dilute acid to improve glucose yield and hydrogen production during dark-fermentation. An optimal glucose yield of 0.95±0.08g/g-trehalose was obtained when trehalose was pretreated by microwave heating with 1% dilute H2SO4 for 25min at 120°C. The hydrolyzed trehalose was inoculated with HPB (hydrogen-producing bacteria) to produce hydrogen during dark-fermentation. The residual solution of dark-fermentation was reused by PSB (photosynthetic bacteria) during photo-fermentation. The residual solution of photo-fermentation was reused by MPB (methane-producing bacteria) during methanogenesis. The maximum yields of hydrogen and methane were 731.3±33.6ml/g-trehalose and 116.9±4.3ml/g-trehalose. The sequential generation of hydrogen and methane from trehalose remarkably enhanced the energy conversion efficiency from 47.2±2.2% in hydrogen fermentation to 72.2±3.1% in combined hydrogen fermentation and methanogenesis.

Original languageEnglish (US)
Pages (from-to)631-637
Number of pages7
StatePublished - Jun 15 2013
Externally publishedYes

Bibliographical note

Funding Information:
This study is supported by National Natural Science Foundation of China ( 51176163 ), International Sci. & Tech. Cooperation Program of China ( 2012DFG61770 , 2010DFA72730 ), National High Technology R&D Program of China ( 2012AA050101 ), National Key Technology R&D Program of China ( 2011BAD14B02 ), Specialized Research Fund for the Doctoral Program of Higher Education ( 20110101110021 ), Program for New Century Excellent Talents in University ( NCET-11-0446 ), Key Natural Science Foundation of Zhejiang Province ( Z1090532 ), and Program of Introducing Talents of Discipline to University ( B08026 ).


  • Dark- and photo-fermentation
  • Hydrogen
  • Methanogenesis
  • Microwave heating
  • Trehalose


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