Column selectivity in reversed-phase liquid chromatography: II. Effect of a change in conditions

N. S. Wilson, M. D. Nelson, J. W. Dolan, L. R. Snyder, P. W. Carr

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148 Scopus citations


The isocratic retention of 67 widely-different solutes in reversed-phase liquid chromatography (RP-LC) has been investigated as a function of temperature and mobile phase composition (% B) for three different C18 columns. Similar studies were also carried out in a gradient mode, where temperature, gradient time and solvent type were varied. These results show that changes in retention with these conditions are similar for each of these three columns. This suggests that relative column selectivity as defined by experiments for one set of experimental conditions will be approximately applicable for other conditions, with the exception of changes in mobile phase pH - which can affect values of the column parameter C (a measure of silanol ionization). Column selectivity as a function of pH was explored for several columns.

Original languageEnglish (US)
Pages (from-to)195-215
Number of pages21
JournalJournal of Chromatography A
Issue number2
StatePublished - Jul 5 2002

Bibliographical note

Copyright 2008 Elsevier B.V., All rights reserved.


  • Column selectivity
  • Mobile phase composition
  • Retention prediction
  • Selectivity
  • Stationary phase, LC
  • Temperature effects

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