Collagenesis and elastogenesis in fibrin-based media-equivalents treated with TGF-ßl and Insulin

Erin D. Grassl, Jennifer L. Long, Jeffrey R. Ross, Brett C. Isenberg, Theodore R. Oegema, Robert T. Tranquillo

Research output: Contribution to conferencePaper

Abstract

The neonatal smooth muscle cells (SMC) produced more collagen when seeded in fibrin gel rather than collagen gel. The quantity of cross-linked elastic fibers was enhanced by treatment with TGFβ1 and insulin. The When compared to collagen ME , the fibrin ME compacted to a greater extent and were both stronger and stiffer when cultured under the same conditions, having a tensile modulus and ultimate tensile strength (UTS). It was found that the use of soluble factors increased elastin production, as measured by alkaline extraction and amino acid analysis, 5-fold in fibrin gels, which was about 4 times greater than in collagen gels.

Original languageEnglish (US)
Number of pages1
StatePublished - Dec 1 2002
EventThird Smith and Nephew International Symposium - Translating Tissue Engineering into Products - Atlanta, GA, United States
Duration: Oct 13 2002Oct 16 2002

Other

OtherThird Smith and Nephew International Symposium - Translating Tissue Engineering into Products
CountryUnited States
CityAtlanta, GA
Period10/13/0210/16/02

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