Colicins B and Ia as novel counterselective agents in interspecies conjugal DNA transfers from colicin-sensitive escherichia coli donors to other gram-negative recipient species

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Abstract

Colicins are powerful counterselective agents in conjugal DNA transfers using colicin-sensitive Escherichia coli donor strains and colicin-resistant recipient species. We have constructed a high-level constitutive expression system for the bacteriocin and immunity genes of channel-forming colicins B and Ia to optimize production of these colicins as molecular biology reagents.

Original languageEnglish (US)
Pages (from-to)39-42
Number of pages4
JournalGene
Volume178
Issue number1-2
DOIs
StatePublished - Oct 31 1996

Keywords

  • Bordetella bronchiseptica
  • Bordetella pertussis
  • Colicin
  • Conjugation
  • Iron
  • Pseudomonas aeruginosa

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