TY - JOUR
T1 - Colicins B and Ia as novel counterselective agents in interspecies conjugal DNA transfers from colicin-sensitive escherichia coli donors to other gram-negative recipient species
AU - Brickman, Timothy J.
AU - Armstrong, Sandra K.
PY - 1996/10/31
Y1 - 1996/10/31
N2 - Colicins are powerful counterselective agents in conjugal DNA transfers using colicin-sensitive Escherichia coli donor strains and colicin-resistant recipient species. We have constructed a high-level constitutive expression system for the bacteriocin and immunity genes of channel-forming colicins B and Ia to optimize production of these colicins as molecular biology reagents.
AB - Colicins are powerful counterselective agents in conjugal DNA transfers using colicin-sensitive Escherichia coli donor strains and colicin-resistant recipient species. We have constructed a high-level constitutive expression system for the bacteriocin and immunity genes of channel-forming colicins B and Ia to optimize production of these colicins as molecular biology reagents.
KW - Bordetella bronchiseptica
KW - Bordetella pertussis
KW - Colicin
KW - Conjugation
KW - Iron
KW - Pseudomonas aeruginosa
UR - http://www.scopus.com/inward/record.url?scp=0030608380&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030608380&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(96)00331-9
DO - 10.1016/0378-1119(96)00331-9
M3 - Article
C2 - 8921889
AN - SCOPUS:0030608380
SN - 0378-1119
VL - 178
SP - 39
EP - 42
JO - Gene
JF - Gene
IS - 1-2
ER -
