Cold sensitive rad52 alleles of yeast

Minh M. Nguyen; Dennis M. Livingston

doi:10.1007/s002940050253

Cold sensitive rad52 alleles of yeast

Minh M. Nguyen
, Dennis M. Livingston

Molecular Biology (TMED)

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

We have characterized two rad52 mutations that are cold-sensitive for growth on MMS agar. The mutations change residues 61 and 69, respectively, in the 504 amino-acid open reading frame. Neither mutation has a profound effect on mitotic crossing-over or on gene conversion. One has a severe deficiency in sporulation at all temperatures, while the other has a partial deficiency and reduced spore viability. Both mutants are retarded in growth on MMS agar by a high-copy plasmid expressing RAD5I.

Original language	English (US)
Pages (from-to)	100-107
Number of pages	8
Journal	Current Genetics
Volume	32
Issue number	2
DOIs	https://doi.org/10.1007/s002940050253
State	Published - 1997

Bibliographical note

Funding Information:
Acknowledgements This work was supported by National Science Foundation grant MCB-9603515. We thank Mike Kaytor for enormous help throughout this work.

Keywords

Mutations
RAD51
RAD52
Yeast

Publisher link

10.1007/s002940050253

Find It

Find It at U of M Libraries

Cite this

@article{e58b169fac5d415fb3724801e05272fd,

title = "Cold sensitive rad52 alleles of yeast",

abstract = "We have characterized two rad52 mutations that are cold-sensitive for growth on MMS agar. The mutations change residues 61 and 69, respectively, in the 504 amino-acid open reading frame. Neither mutation has a profound effect on mitotic crossing-over or on gene conversion. One has a severe deficiency in sporulation at all temperatures, while the other has a partial deficiency and reduced spore viability. Both mutants are retarded in growth on MMS agar by a high-copy plasmid expressing RAD5I.",

keywords = "Mutations, RAD51, RAD52, Yeast",

author = "Nguyen, \{Minh M.\} and Livingston, \{Dennis M.\}",

note = "Funding Information: Acknowledgements This work was supported by National Science Foundation grant MCB-9603515. We thank Mike Kaytor for enormous help throughout this work.",

year = "1997",

doi = "10.1007/s002940050253",

language = "English (US)",

volume = "32",

pages = "100--107",

journal = "Current Genetics",

issn = "0172-8083",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "2",

}

TY - JOUR

T1 - Cold sensitive rad52 alleles of yeast

AU - Nguyen, Minh M.

AU - Livingston, Dennis M.

N1 - Funding Information: Acknowledgements This work was supported by National Science Foundation grant MCB-9603515. We thank Mike Kaytor for enormous help throughout this work.

PY - 1997

Y1 - 1997

N2 - We have characterized two rad52 mutations that are cold-sensitive for growth on MMS agar. The mutations change residues 61 and 69, respectively, in the 504 amino-acid open reading frame. Neither mutation has a profound effect on mitotic crossing-over or on gene conversion. One has a severe deficiency in sporulation at all temperatures, while the other has a partial deficiency and reduced spore viability. Both mutants are retarded in growth on MMS agar by a high-copy plasmid expressing RAD5I.

AB - We have characterized two rad52 mutations that are cold-sensitive for growth on MMS agar. The mutations change residues 61 and 69, respectively, in the 504 amino-acid open reading frame. Neither mutation has a profound effect on mitotic crossing-over or on gene conversion. One has a severe deficiency in sporulation at all temperatures, while the other has a partial deficiency and reduced spore viability. Both mutants are retarded in growth on MMS agar by a high-copy plasmid expressing RAD5I.

KW - Mutations

KW - RAD51

KW - RAD52

KW - Yeast

UR - https://www.scopus.com/pages/publications/0030844022

UR - https://www.scopus.com/pages/publications/0030844022#tab=citedBy

U2 - 10.1007/s002940050253

DO - 10.1007/s002940050253

M3 - Article

C2 - 9294257

AN - SCOPUS:0030844022

SN - 0172-8083

VL - 32

SP - 100

EP - 107

JO - Current Genetics

JF - Current Genetics

IS - 2

ER -

Cold sensitive rad52 alleles of yeast

Abstract

Bibliographical note

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this