Cold sensitive rad52 alleles of yeast

Minh M. Nguyen, Dennis M. Livingston

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

We have characterized two rad52 mutations that are cold-sensitive for growth on MMS agar. The mutations change residues 61 and 69, respectively, in the 504 amino-acid open reading frame. Neither mutation has a profound effect on mitotic crossing-over or on gene conversion. One has a severe deficiency in sporulation at all temperatures, while the other has a partial deficiency and reduced spore viability. Both mutants are retarded in growth on MMS agar by a high-copy plasmid expressing RAD5I.

Original languageEnglish (US)
Pages (from-to)100-107
Number of pages8
JournalCurrent Genetics
Volume32
Issue number2
DOIs
StatePublished - 1997

Bibliographical note

Funding Information:
Acknowledgements This work was supported by National Science Foundation grant MCB-9603515. We thank Mike Kaytor for enormous help throughout this work.

Keywords

  • Mutations
  • RAD51
  • RAD52
  • Yeast

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