We have previously shown that the Xenopus homologue of cold-inducible RNA binding protein, XCIRP-1, is required for the morphogenetic migration of the pronephros during embryonic development. However, the underlying molecular mechanisms remain elusive. Here, we report that XCIRP is essential for embryonic cell movement, as suppression of XCIRP by microinjection of anti-sense mRNA and morpholino antisense oligonucleotides (MOs) significantly reduced protein expression, inhibited the cell migration rate, and inhibited eFGF and activin-induced animal cap elongation. By immunoprecipitation and RT-PCR, we further showed that the mRNA of a panel of adhesion molecules, including αE- and β-catenin, C- and E-cadherin, and paraxial proto-cadherin, are the targets of XCIRP. Consistently, in animal cap explant studies, suppression of XCIRP by MOs inhibited the expression of these adhesion molecules, while over-expression of sense XCIRP-1 mRNA fully rescued this inhibition. Taken together, these results suggest for the first time that XCIRP is required to maintain the expression of adhesion molecules and cell movement during embryonic development.
|Original language||English (US)|
|Number of pages||9|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - May 26 2006|
Bibliographical noteFunding Information:
The work described in this paper was supported by a grant from the Research Grants Council of the Hong Kong Special Administrative Region, China (HKU 7198/01M to M.C. Lin and CUHK7328/04M to H.-F. Kung); by Li Ka Shing Institute of Health Sciences and by a grant from the Chinese National Natural Science Fund (39870267 to Y. Peng).
- Cell movement
- Cold-inducible RNA binding protein
- Morphogenetic lineage migration
- Neural development