Cold-inducible RNA binding protein is required for the expression of adhesion molecules and embryonic cell movement in Xenopus laevis

Ying Peng, Pai Hao Yang, Julian A. Tanner, Jian Dong Huang, Ming Li, Henry F. Lee, Ren He Xu, Hsiang Fu Kung, Marie C.M. Lin

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

We have previously shown that the Xenopus homologue of cold-inducible RNA binding protein, XCIRP-1, is required for the morphogenetic migration of the pronephros during embryonic development. However, the underlying molecular mechanisms remain elusive. Here, we report that XCIRP is essential for embryonic cell movement, as suppression of XCIRP by microinjection of anti-sense mRNA and morpholino antisense oligonucleotides (MOs) significantly reduced protein expression, inhibited the cell migration rate, and inhibited eFGF and activin-induced animal cap elongation. By immunoprecipitation and RT-PCR, we further showed that the mRNA of a panel of adhesion molecules, including αE- and β-catenin, C- and E-cadherin, and paraxial proto-cadherin, are the targets of XCIRP. Consistently, in animal cap explant studies, suppression of XCIRP by MOs inhibited the expression of these adhesion molecules, while over-expression of sense XCIRP-1 mRNA fully rescued this inhibition. Taken together, these results suggest for the first time that XCIRP is required to maintain the expression of adhesion molecules and cell movement during embryonic development.

Original languageEnglish (US)
Pages (from-to)416-424
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume344
Issue number1
DOIs
StatePublished - May 26 2006
Externally publishedYes

Bibliographical note

Funding Information:
The work described in this paper was supported by a grant from the Research Grants Council of the Hong Kong Special Administrative Region, China (HKU 7198/01M to M.C. Lin and CUHK7328/04M to H.-F. Kung); by Li Ka Shing Institute of Health Sciences and by a grant from the Chinese National Natural Science Fund (39870267 to Y. Peng).

Keywords

  • Cell movement
  • Cold-inducible RNA binding protein
  • Morphogenetic lineage migration
  • Neural development
  • Xenopus

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