Postmortem examination of the cochlea with a cochlear implant in the scala tympani presents several challenges. It is technologically difficult to section a cochlea with an implant due to the presence of its wires and metal components that are adjacent to the membranous and bony tissues of the cochlea. These metal components damage traditional steel blades of a microtome in celloidin, paraffin or frozen embedded tissues. However, plastic embedded implanted cochleas have been successfully sectioned using specialized methods (Irving et al., 2013). An alternative non-destructive method is to optically section a chemically cleared cochlea using light-sheet microscopy, which we will describe in this publication. However, since this method uses a light-sheet to section the cochlea the opaque and reflective metal components of the implant results in some artifacts in the 2D optical sections. The best image quality using light-sheet fluorescent microscopy is when the implant is removed prior to imaging.
|Original language||English (US)|
|State||Published - Dec 2022|
Bibliographical noteFunding Information:
The authors thank Alec Brown, Luke DeMars, and Jacob Loosen for 3D imaging in Amira, Julian Wüster for design and construction of a rotating holder for sTSLIM, and Thomas Pengo for writing the program to convert the rotational stacks to translational stacks. Funding has been provided the Cochlear Americas, the NIDCD (RO1DC007588–04), and private donations from Bridget Sperl and John McCormick, and the Kellogg Foundation.
© 2022 Elsevier B.V.
- Cochlear implant
- Guinea pig
- Light-sheet microscopy
PubMed: MeSH publication types
- Journal Article
- Research Support, N.I.H., Extramural
- Research Support, Non-U.S. Gov't