Cochlear implant imaging in the mouse and guinea pig using light-sheet microscopy

Peter A. Santi; Shane Johnson; Julian Wüster; Matthew Griesbach; Alexander Claussen; Jonathon Kirk

doi:10.1016/j.heares.2022.108639

Cochlear implant imaging in the mouse and guinea pig using light-sheet microscopy

Peter A. Santi, Shane Johnson, Julian Wüster, Matthew Griesbach, Alexander Claussen, Jonathon Kirk

Otolaryngology

Research output: Contribution to journal › Review article › peer-review

Abstract

Postmortem examination of the cochlea with a cochlear implant in the scala tympani presents several challenges. It is technologically difficult to section a cochlea with an implant due to the presence of its wires and metal components that are adjacent to the membranous and bony tissues of the cochlea. These metal components damage traditional steel blades of a microtome in celloidin, paraffin or frozen embedded tissues. However, plastic embedded implanted cochleas have been successfully sectioned using specialized methods (Irving et al., 2013). An alternative non-destructive method is to optically section a chemically cleared cochlea using light-sheet microscopy, which we will describe in this publication. However, since this method uses a light-sheet to section the cochlea the opaque and reflective metal components of the implant results in some artifacts in the 2D optical sections. The best image quality using light-sheet fluorescent microscopy is when the implant is removed prior to imaging.

Original language	English (US)
Article number	108639
Journal	Hearing Research
Volume	426
DOIs	https://doi.org/10.1016/j.heares.2022.108639
State	Published - Dec 2022

Bibliographical note

Funding Information:
The authors thank Alec Brown, Luke DeMars, and Jacob Loosen for 3D imaging in Amira, Julian Wüster for design and construction of a rotating holder for sTSLIM, and Thomas Pengo for writing the program to convert the rotational stacks to translational stacks. Funding has been provided the Cochlear Americas, the NIDCD (RO1DC007588–04), and private donations from Bridget Sperl and John McCormick, and the Kellogg Foundation.

Publisher Copyright:
© 2022 Elsevier B.V.

Keywords

Cochlea
Cochlear implant
Guinea pig
Light-sheet microscopy
Mouse

PubMed: MeSH publication types

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

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10.1016/j.heares.2022.108639

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title = "Cochlear implant imaging in the mouse and guinea pig using light-sheet microscopy",

abstract = "Postmortem examination of the cochlea with a cochlear implant in the scala tympani presents several challenges. It is technologically difficult to section a cochlea with an implant due to the presence of its wires and metal components that are adjacent to the membranous and bony tissues of the cochlea. These metal components damage traditional steel blades of a microtome in celloidin, paraffin or frozen embedded tissues. However, plastic embedded implanted cochleas have been successfully sectioned using specialized methods (Irving et al., 2013). An alternative non-destructive method is to optically section a chemically cleared cochlea using light-sheet microscopy, which we will describe in this publication. However, since this method uses a light-sheet to section the cochlea the opaque and reflective metal components of the implant results in some artifacts in the 2D optical sections. The best image quality using light-sheet fluorescent microscopy is when the implant is removed prior to imaging.",

keywords = "Cochlea, Cochlear implant, Guinea pig, Light-sheet microscopy, Mouse",

author = "Santi, {Peter A.} and Shane Johnson and Julian W{\"u}ster and Matthew Griesbach and Alexander Claussen and Jonathon Kirk",

note = "Funding Information: The authors thank Alec Brown, Luke DeMars, and Jacob Loosen for 3D imaging in Amira, Julian W{\"u}ster for design and construction of a rotating holder for sTSLIM, and Thomas Pengo for writing the program to convert the rotational stacks to translational stacks. Funding has been provided the Cochlear Americas, the NIDCD (RO1DC007588–04), and private donations from Bridget Sperl and John McCormick, and the Kellogg Foundation. Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",

year = "2022",

month = dec,

doi = "10.1016/j.heares.2022.108639",

language = "English (US)",

volume = "426",

journal = "Hearing Research",

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T1 - Cochlear implant imaging in the mouse and guinea pig using light-sheet microscopy

AU - Santi, Peter A.

AU - Johnson, Shane

AU - Wüster, Julian

AU - Griesbach, Matthew

AU - Claussen, Alexander

AU - Kirk, Jonathon

N1 - Funding Information: The authors thank Alec Brown, Luke DeMars, and Jacob Loosen for 3D imaging in Amira, Julian Wüster for design and construction of a rotating holder for sTSLIM, and Thomas Pengo for writing the program to convert the rotational stacks to translational stacks. Funding has been provided the Cochlear Americas, the NIDCD (RO1DC007588–04), and private donations from Bridget Sperl and John McCormick, and the Kellogg Foundation. Publisher Copyright: © 2022 Elsevier B.V.

PY - 2022/12

Y1 - 2022/12

N2 - Postmortem examination of the cochlea with a cochlear implant in the scala tympani presents several challenges. It is technologically difficult to section a cochlea with an implant due to the presence of its wires and metal components that are adjacent to the membranous and bony tissues of the cochlea. These metal components damage traditional steel blades of a microtome in celloidin, paraffin or frozen embedded tissues. However, plastic embedded implanted cochleas have been successfully sectioned using specialized methods (Irving et al., 2013). An alternative non-destructive method is to optically section a chemically cleared cochlea using light-sheet microscopy, which we will describe in this publication. However, since this method uses a light-sheet to section the cochlea the opaque and reflective metal components of the implant results in some artifacts in the 2D optical sections. The best image quality using light-sheet fluorescent microscopy is when the implant is removed prior to imaging.

AB - Postmortem examination of the cochlea with a cochlear implant in the scala tympani presents several challenges. It is technologically difficult to section a cochlea with an implant due to the presence of its wires and metal components that are adjacent to the membranous and bony tissues of the cochlea. These metal components damage traditional steel blades of a microtome in celloidin, paraffin or frozen embedded tissues. However, plastic embedded implanted cochleas have been successfully sectioned using specialized methods (Irving et al., 2013). An alternative non-destructive method is to optically section a chemically cleared cochlea using light-sheet microscopy, which we will describe in this publication. However, since this method uses a light-sheet to section the cochlea the opaque and reflective metal components of the implant results in some artifacts in the 2D optical sections. The best image quality using light-sheet fluorescent microscopy is when the implant is removed prior to imaging.

KW - Cochlea

KW - Cochlear implant

KW - Guinea pig

KW - Light-sheet microscopy

KW - Mouse

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DO - 10.1016/j.heares.2022.108639

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JO - Hearing Research

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Cochlear implant imaging in the mouse and guinea pig using light-sheet microscopy

Abstract

Bibliographical note

Keywords

PubMed: MeSH publication types

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