TY - JOUR
T1 - Cocaine-mediated suppression of superoxide production by human peripheral blood mononuclear cells
AU - Chao, C. C.
AU - Molitor, T. W.
AU - Gekker, G.
AU - Murtaugh, P.
AU - Peterson, P. K.
PY - 1991
Y1 - 1991
N2 - Cocaine, like opiates, modulates a variety of immune functions. In the present study, we investigated the effect of cocaine on superoxide anion (O-2) production, an index of a microbicidal activity, by cultured human peripheral blood mononuclear cells. Release of O-2 was measured by superoxide dismutase-inhibitable reduction of ferricytochrome C in response to phorbolmyristate acetate. Peripheral blood mononuclear cells cultured in the presence of cocaine (1 μM) for 48 hr released less (P<.05) O-2 than did nontreated control cells (95.1 ± 10.2 vs. 57.9 ± 6.6 nmol/107 cells/60 min, respectively). This suppressive effect was dose-dependent. Antibodies to transforming growth factor-beta, a cytokine inhibitory of monocyte O-2 production, abrogated (P<.01 ) cocaine-mediated suppression, suggesting that transforming growth factor-beta is involved in the suppression. Also, naloxone blocked (P<.01) the suppressive effects of both cocaine and transforming growth factor-beta on O-2 production, suggesting that the suppressive mechanism is naloxone-sensitive.
AB - Cocaine, like opiates, modulates a variety of immune functions. In the present study, we investigated the effect of cocaine on superoxide anion (O-2) production, an index of a microbicidal activity, by cultured human peripheral blood mononuclear cells. Release of O-2 was measured by superoxide dismutase-inhibitable reduction of ferricytochrome C in response to phorbolmyristate acetate. Peripheral blood mononuclear cells cultured in the presence of cocaine (1 μM) for 48 hr released less (P<.05) O-2 than did nontreated control cells (95.1 ± 10.2 vs. 57.9 ± 6.6 nmol/107 cells/60 min, respectively). This suppressive effect was dose-dependent. Antibodies to transforming growth factor-beta, a cytokine inhibitory of monocyte O-2 production, abrogated (P<.01 ) cocaine-mediated suppression, suggesting that transforming growth factor-beta is involved in the suppression. Also, naloxone blocked (P<.01) the suppressive effects of both cocaine and transforming growth factor-beta on O-2 production, suggesting that the suppressive mechanism is naloxone-sensitive.
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M3 - Article
C2 - 1846416
AN - SCOPUS:0026019760
SN - 0022-3565
VL - 256
SP - 255
EP - 258
JO - Journal of Pharmacology and Experimental Therapeutics
JF - Journal of Pharmacology and Experimental Therapeutics
IS - 1
ER -