Cocaine-induced HIV-1 expression in microglia involves sigma-1 receptors and transforming growth factor-β1

Genya Gekker; Shuxian Hu; Wen Sheng; R B Rock; James R Lokensgard; Phillip K. Peterson

doi:10.1016/j.intimp.2005.12.005

Cocaine-induced HIV-1 expression in microglia involves sigma-1 receptors and transforming growth factor-β1

Genya Gekker, Shuxian Hu, Wen Sheng, R B Rock, James R Lokensgard, Phillip K. Peterson

Research output: Contribution to journal › Article › peer-review

73 Scopus citations

Abstract

The neuropharmacological properties of cocaine are known to be associated with the activation of sigma-1 receptors. Cocaine also has been shown to alter both cytokine production and HIV-1 expression in mononuclear phagocytes, including microglial cells. This study tested the hypothesis that sigma-1 receptors and transforming growth factor (TGF)-β1 are involved in cocaine-induced up-regulation of HIV-1 expression in microglial cell cultures. Treatment of microglial cells with cocaine resulted in a concentration-dependent increase in viral expression assessed by measurement of p24 antigen levels in culture supernatants. This cocaine-mediated stimulation of HIV-1 expression was blocked by treatment of microglia with inhibitors of sigma-1 receptors (BD1047) and TGF-β1 (SB-431542 and anti-TGF-β1 antibodies). Microglia were also shown to constitutively express sigma-1 receptor mRNA. Thus, the results of this study support the notion that neuroimmunopharmacological properties of cocaine involve sigma-1 receptors and cytokines.

Original language	English (US)
Pages (from-to)	1029-1033
Number of pages	5
Journal	International Immunopharmacology
Volume	6
Issue number	6
DOIs	https://doi.org/10.1016/j.intimp.2005.12.005
State	Published - Jun 2006

Bibliographical note

Funding Information:
This work was supported by U.S. Public Health Service grant DA-09789. The authors are grateful to Dr. Fred Kravitz for his invaluable assistance.

Keywords

Cocaine
HIV-1
Microglia
Sigma-1 receptor
Transforming growth factor-β1

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.intimp.2005.12.005

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title = "Cocaine-induced HIV-1 expression in microglia involves sigma-1 receptors and transforming growth factor-β1",

abstract = "The neuropharmacological properties of cocaine are known to be associated with the activation of sigma-1 receptors. Cocaine also has been shown to alter both cytokine production and HIV-1 expression in mononuclear phagocytes, including microglial cells. This study tested the hypothesis that sigma-1 receptors and transforming growth factor (TGF)-β1 are involved in cocaine-induced up-regulation of HIV-1 expression in microglial cell cultures. Treatment of microglial cells with cocaine resulted in a concentration-dependent increase in viral expression assessed by measurement of p24 antigen levels in culture supernatants. This cocaine-mediated stimulation of HIV-1 expression was blocked by treatment of microglia with inhibitors of sigma-1 receptors (BD1047) and TGF-β1 (SB-431542 and anti-TGF-β1 antibodies). Microglia were also shown to constitutively express sigma-1 receptor mRNA. Thus, the results of this study support the notion that neuroimmunopharmacological properties of cocaine involve sigma-1 receptors and cytokines.",

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author = "Genya Gekker and Shuxian Hu and Wen Sheng and Rock, {R B} and Lokensgard, {James R} and Peterson, {Phillip K.}",

note = "Funding Information: This work was supported by U.S. Public Health Service grant DA-09789. The authors are grateful to Dr. Fred Kravitz for his invaluable assistance.",

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AU - Sheng, Wen

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AU - Lokensgard, James R

AU - Peterson, Phillip K.

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N2 - The neuropharmacological properties of cocaine are known to be associated with the activation of sigma-1 receptors. Cocaine also has been shown to alter both cytokine production and HIV-1 expression in mononuclear phagocytes, including microglial cells. This study tested the hypothesis that sigma-1 receptors and transforming growth factor (TGF)-β1 are involved in cocaine-induced up-regulation of HIV-1 expression in microglial cell cultures. Treatment of microglial cells with cocaine resulted in a concentration-dependent increase in viral expression assessed by measurement of p24 antigen levels in culture supernatants. This cocaine-mediated stimulation of HIV-1 expression was blocked by treatment of microglia with inhibitors of sigma-1 receptors (BD1047) and TGF-β1 (SB-431542 and anti-TGF-β1 antibodies). Microglia were also shown to constitutively express sigma-1 receptor mRNA. Thus, the results of this study support the notion that neuroimmunopharmacological properties of cocaine involve sigma-1 receptors and cytokines.

AB - The neuropharmacological properties of cocaine are known to be associated with the activation of sigma-1 receptors. Cocaine also has been shown to alter both cytokine production and HIV-1 expression in mononuclear phagocytes, including microglial cells. This study tested the hypothesis that sigma-1 receptors and transforming growth factor (TGF)-β1 are involved in cocaine-induced up-regulation of HIV-1 expression in microglial cell cultures. Treatment of microglial cells with cocaine resulted in a concentration-dependent increase in viral expression assessed by measurement of p24 antigen levels in culture supernatants. This cocaine-mediated stimulation of HIV-1 expression was blocked by treatment of microglia with inhibitors of sigma-1 receptors (BD1047) and TGF-β1 (SB-431542 and anti-TGF-β1 antibodies). Microglia were also shown to constitutively express sigma-1 receptor mRNA. Thus, the results of this study support the notion that neuroimmunopharmacological properties of cocaine involve sigma-1 receptors and cytokines.

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KW - Microglia

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KW - Transforming growth factor-β1

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Cocaine-induced HIV-1 expression in microglia involves sigma-1 receptors and transforming growth factor-β1

Abstract

Bibliographical note

Keywords

UN SDGs

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