Coat protein-mediated resistance to pea enation mosaic virus in transgenic Pisum sativum L.

Gangamma M. Chowrira, Timothy D. Cavileer, Sanjay K. Gupta, Paul F. Lurquin, Philip H. Berger

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Pea (Pisum sativum L.) plants were transformed in planta by injection/electroporation of axillary meristems with a chimeric pea enation mosaic virus (PEMV) coat protein gene contruct. R1 progenies of these plants were shown to harbor the transgene by polymerase chain reaction (PCR) and genomic Southern analysis, while transgene expression was demonstrated by western blot analysis. Transgenic R2, R3 and R4 plants displayed delayed or transient PEMV multiplication and attenuated symptoms as compated to control inoculated individuals.

Original languageEnglish (US)
Pages (from-to)265-271
Number of pages7
JournalTransgenic Research
Volume7
Issue number4
DOIs
StatePublished - Jul 1 1998
Externally publishedYes

Keywords

  • Coat protein-mediated virus resistance
  • PEMV, pea enation mosaic virus, electroporation
  • Pea transformation
  • Transgenic plants

Fingerprint Dive into the research topics of 'Coat protein-mediated resistance to pea enation mosaic virus in transgenic Pisum sativum L.'. Together they form a unique fingerprint.

Cite this