Pea (Pisum sativum L.) plants were transformed in planta by injection/electroporation of axillary meristems with a chimeric pea enation mosaic virus (PEMV) coat protein gene contruct. R1 progenies of these plants were shown to harbor the transgene by polymerase chain reaction (PCR) and genomic Southern analysis, while transgene expression was demonstrated by western blot analysis. Transgenic R2, R3 and R4 plants displayed delayed or transient PEMV multiplication and attenuated symptoms as compated to control inoculated individuals.
Bibliographical noteFunding Information:
The authors thank Dr G. de Zoeten for providing the PEMV-CP antiserum and cDNA clone, Steve Wyatt for his helpful discussions, and Patrick Shiel for excellent technical assistance. This work was supported by grants from the Dry Pea & Lentil Commission of Washington, the USDA=Cool Season Food Legume Research Program, the Northwest Agricultural Research Foundation, and the WSU Summer Graduate Research Assistantship awarded to G.M. Chowrira during Summer 1995.
- Coat protein-mediated virus resistance
- PEMV, pea enation mosaic virus, electroporation
- Pea transformation
- Transgenic plants