Coat protein-mediated resistance to pea enation mosaic virus in transgenic Pisum sativum L.

Gangamma M. Chowrira; Timothy D. Cavileer; Sanjay K. Gupta; Paul F. Lurquin; Philip H. Berger

doi:10.1023/A:1008874129407

Coat protein-mediated resistance to pea enation mosaic virus in transgenic Pisum sativum L.

Gangamma M. Chowrira, Timothy D. Cavileer, Sanjay K. Gupta, Paul F. Lurquin, Philip H. Berger

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Pea (Pisum sativum L.) plants were transformed in planta by injection/electroporation of axillary meristems with a chimeric pea enation mosaic virus (PEMV) coat protein gene contruct. R₁ progenies of these plants were shown to harbor the transgene by polymerase chain reaction (PCR) and genomic Southern analysis, while transgene expression was demonstrated by western blot analysis. Transgenic R₂, R₃ and R₄ plants displayed delayed or transient PEMV multiplication and attenuated symptoms as compated to control inoculated individuals.

Original language	English (US)
Pages (from-to)	265-271
Number of pages	7
Journal	Transgenic Research
Volume	7
Issue number	4
DOIs	https://doi.org/10.1023/A:1008874129407
State	Published - Jul 1998
Externally published	Yes

Bibliographical note

Funding Information:
The authors thank Dr G. de Zoeten for providing the PEMV-CP antiserum and cDNA clone, Steve Wyatt for his helpful discussions, and Patrick Shiel for excellent technical assistance. This work was supported by grants from the Dry Pea & Lentil Commission of Washington, the USDA=Cool Season Food Legume Research Program, the Northwest Agricultural Research Foundation, and the WSU Summer Graduate Research Assistantship awarded to G.M. Chowrira during Summer 1995.

Keywords

Coat protein-mediated virus resistance
PEMV, pea enation mosaic virus, electroporation
Pea transformation
Transgenic plants

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10.1023/A:1008874129407

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title = "Coat protein-mediated resistance to pea enation mosaic virus in transgenic Pisum sativum L.",

abstract = "Pea (Pisum sativum L.) plants were transformed in planta by injection/electroporation of axillary meristems with a chimeric pea enation mosaic virus (PEMV) coat protein gene contruct. R1 progenies of these plants were shown to harbor the transgene by polymerase chain reaction (PCR) and genomic Southern analysis, while transgene expression was demonstrated by western blot analysis. Transgenic R2, R3 and R4 plants displayed delayed or transient PEMV multiplication and attenuated symptoms as compated to control inoculated individuals.",

keywords = "Coat protein-mediated virus resistance, PEMV, pea enation mosaic virus, electroporation, Pea transformation, Transgenic plants",

author = "Chowrira, {Gangamma M.} and Cavileer, {Timothy D.} and Gupta, {Sanjay K.} and Lurquin, {Paul F.} and Berger, {Philip H.}",

note = "Funding Information: The authors thank Dr G. de Zoeten for providing the PEMV-CP antiserum and cDNA clone, Steve Wyatt for his helpful discussions, and Patrick Shiel for excellent technical assistance. This work was supported by grants from the Dry Pea & Lentil Commission of Washington, the USDA=Cool Season Food Legume Research Program, the Northwest Agricultural Research Foundation, and the WSU Summer Graduate Research Assistantship awarded to G.M. Chowrira during Summer 1995.",

year = "1998",

month = jul,

doi = "10.1023/A:1008874129407",

language = "English (US)",

volume = "7",

pages = "265--271",

journal = "Transgenic Research",

issn = "0962-8819",

publisher = "Springer Netherlands",

number = "4",

}

TY - JOUR

T1 - Coat protein-mediated resistance to pea enation mosaic virus in transgenic Pisum sativum L.

AU - Chowrira, Gangamma M.

AU - Cavileer, Timothy D.

AU - Gupta, Sanjay K.

AU - Lurquin, Paul F.

AU - Berger, Philip H.

N1 - Funding Information: The authors thank Dr G. de Zoeten for providing the PEMV-CP antiserum and cDNA clone, Steve Wyatt for his helpful discussions, and Patrick Shiel for excellent technical assistance. This work was supported by grants from the Dry Pea & Lentil Commission of Washington, the USDA=Cool Season Food Legume Research Program, the Northwest Agricultural Research Foundation, and the WSU Summer Graduate Research Assistantship awarded to G.M. Chowrira during Summer 1995.

PY - 1998/7

Y1 - 1998/7

N2 - Pea (Pisum sativum L.) plants were transformed in planta by injection/electroporation of axillary meristems with a chimeric pea enation mosaic virus (PEMV) coat protein gene contruct. R1 progenies of these plants were shown to harbor the transgene by polymerase chain reaction (PCR) and genomic Southern analysis, while transgene expression was demonstrated by western blot analysis. Transgenic R2, R3 and R4 plants displayed delayed or transient PEMV multiplication and attenuated symptoms as compated to control inoculated individuals.

AB - Pea (Pisum sativum L.) plants were transformed in planta by injection/electroporation of axillary meristems with a chimeric pea enation mosaic virus (PEMV) coat protein gene contruct. R1 progenies of these plants were shown to harbor the transgene by polymerase chain reaction (PCR) and genomic Southern analysis, while transgene expression was demonstrated by western blot analysis. Transgenic R2, R3 and R4 plants displayed delayed or transient PEMV multiplication and attenuated symptoms as compated to control inoculated individuals.

KW - Coat protein-mediated virus resistance

KW - PEMV, pea enation mosaic virus, electroporation

KW - Pea transformation

KW - Transgenic plants

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DO - 10.1023/A:1008874129407

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AN - SCOPUS:0032124758

SN - 0962-8819

VL - 7

SP - 265

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JO - Transgenic Research

JF - Transgenic Research

IS - 4

ER -

Coat protein-mediated resistance to pea enation mosaic virus in transgenic Pisum sativum L.

Abstract

Bibliographical note

Keywords

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