Coagulopathy in α-galactosyl transferase knockout pulmonary xenotransplants

Errol L. Bush, Andrew S. Barbas, Zoie E. Holzknecht, Guerard W. Byrne, Christopher G. McGregor, William Parker, R. Duane Davis, Shu S. Lin

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Background: After substantial progress on many fronts, one of the remaining barriers still opposing the clinical application of xenotransplantation is a disseminated intravascular coagulopathy (DIC) that is observed in the pre-clinical model of porcine-to-primate transplantation. The onset of DIC is particularly rapid in recipients of pulmonary xenografts, usually occurring within the first days or even hours of reperfusion. Methods: In this study, we describe the results of two porcine-to-baboon transplants utilizing porcine lungs depleted of macrophages, deficient in the α-1,3- galactosyltransferase gene, and with the expression of human decay-accelerating factor, a complement regulatory protein. Results: In both cases, evidence of DIC was observed within 48 h of reperfusion, with thrombocytopenia and increases in levels of thrombin-antithrombin complex evident in both cases. Depletion of fibrinogen was observed in one graft, whereas elevation of D-dimer levels was observed in the other. One graft, which showed focal lymphocytic infiltrates pre-operatively, failed within 3 h. Conclusions: The results indicate that further efforts to address the coagulopathy associated with pulmonary xenotransplantation are needed. Further, evidence suggests that resident porcine immune cells can play an important role in the coagulopathy associated with xenotransplantation.

Original languageEnglish (US)
Pages (from-to)6-13
Number of pages8
Issue number1
StatePublished - Jan 2011
Externally publishedYes


  • 1,3-galactosyltransferase
  • baboon
  • coagulopathy
  • macrophage
  • porcine
  • pre-clinical
  • pulmonary
  • xenotransplant


Dive into the research topics of 'Coagulopathy in α-galactosyl transferase knockout pulmonary xenotransplants'. Together they form a unique fingerprint.

Cite this