TY - JOUR
T1 - Co-Inoculation with Azospirillum brasilense and Bradyrhizobium sp. Enhances Nitrogen Uptake and Yield in Field-Grown Cowpea and Did Not Change N-Fertilizer Recovery
AU - Galindo, Fernando Shintate
AU - Pagliari, Paulo Humberto
AU - da Silva, Edson Cabral
AU - Silva, Vinicius Martins
AU - Fernandes, Guilherme Carlos
AU - Rodrigues, Willian Lima
AU - Céu, Elaine Garcia Oliveira
AU - de Lima, Bruno Horschut
AU - Jalal, Arshad
AU - Muraoka, Takashi
AU - Buzetti, Salatiér
AU - Lavres, José
AU - Teixeira Filho, Marcelo Carvalho Minhoto
N1 - Funding Information:
This research was funded by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), grant number 2018/08485-7 and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, award number 312359/2017-9).
Publisher Copyright:
© 2022 by the authors.
PY - 2022/7
Y1 - 2022/7
N2 - This study was designed to investigate the effects of Azospirillum brasilense and Bradyrhizobium sp. co-inoculation coupled with N application on soil N levels and N in plants (total N, nitrate N-NO3− and ammonium N-NH4+), photosynthetic pigments, cowpea plant biomass and grain yield. An isotopic technique was employed to evaluate 15N fertilizer recovery and derivation. Field trials involved two inoculations—(i) single Bradyrhizobium sp. and (ii) Bradyrhizobium sp. + A. brasilense co-inoculation—and four N fertilizer rates (0, 20, 40 and 80 kg ha−1). The co-inoculation of Bradyrhizobium sp. + A. brasilense increased cowpea N uptake (an increase from 10 to 14%) and grain yield (an average increase of 8%) compared to the standard inoculation with Bradyrhizobium sp. specifically derived from soil and other sources without affecting 15N fertilizer recovery. There is no need for the supplementation of N via mineral fertilizers when A. brasilense co-inoculation is performed in a cowpea crop. However, even in the case of an NPK basal fertilization, applied N rates should remain below 20 kg N ha−1 when co-inoculation with Bradyrhizobium sp. and A. brasilense is performed.
AB - This study was designed to investigate the effects of Azospirillum brasilense and Bradyrhizobium sp. co-inoculation coupled with N application on soil N levels and N in plants (total N, nitrate N-NO3− and ammonium N-NH4+), photosynthetic pigments, cowpea plant biomass and grain yield. An isotopic technique was employed to evaluate 15N fertilizer recovery and derivation. Field trials involved two inoculations—(i) single Bradyrhizobium sp. and (ii) Bradyrhizobium sp. + A. brasilense co-inoculation—and four N fertilizer rates (0, 20, 40 and 80 kg ha−1). The co-inoculation of Bradyrhizobium sp. + A. brasilense increased cowpea N uptake (an increase from 10 to 14%) and grain yield (an average increase of 8%) compared to the standard inoculation with Bradyrhizobium sp. specifically derived from soil and other sources without affecting 15N fertilizer recovery. There is no need for the supplementation of N via mineral fertilizers when A. brasilense co-inoculation is performed in a cowpea crop. However, even in the case of an NPK basal fertilization, applied N rates should remain below 20 kg N ha−1 when co-inoculation with Bradyrhizobium sp. and A. brasilense is performed.
KW - Vigna unguiculata (L.) Walp
KW - biological nitrogen fixation
KW - nitrogen fractions
KW - nitrogen-
KW - plant growth rhizobacteria
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U2 - 10.3390/plants11141847
DO - 10.3390/plants11141847
M3 - Article
C2 - 35890481
AN - SCOPUS:85136406176
SN - 2223-7747
VL - 11
JO - Plants
JF - Plants
IS - 14
M1 - 1847
ER -
