Co-Inoculation with Azospirillum brasilense and Bradyrhizobium sp. Enhances Nitrogen Uptake and Yield in Field-Grown Cowpea and Did Not Change N-Fertilizer Recovery

Fernando Shintate Galindo, Paulo Humberto Pagliari, Edson Cabral da Silva, Vinicius Martins Silva, Guilherme Carlos Fernandes, Willian Lima Rodrigues, Elaine Garcia Oliveira Céu, Bruno Horschut de Lima, Arshad Jalal, Takashi Muraoka, Salatiér Buzetti, José Lavres, Marcelo Carvalho Minhoto Teixeira Filho

Research output: Contribution to journalArticlepeer-review

Abstract

This study was designed to investigate the effects of Azospirillum brasilense and Bradyrhizobium sp. co-inoculation coupled with N application on soil N levels and N in plants (total N, nitrate N-NO3 and ammonium N-NH4+), photosynthetic pigments, cowpea plant biomass and grain yield. An isotopic technique was employed to evaluate 15N fertilizer recovery and derivation. Field trials involved two inoculations—(i) single Bradyrhizobium sp. and (ii) Bradyrhizobium sp. + A. brasilense co-inoculation—and four N fertilizer rates (0, 20, 40 and 80 kg ha−1). The co-inoculation of Bradyrhizobium sp. + A. brasilense increased cowpea N uptake (an increase from 10 to 14%) and grain yield (an average increase of 8%) compared to the standard inoculation with Bradyrhizobium sp. specifically derived from soil and other sources without affecting 15N fertilizer recovery. There is no need for the supplementation of N via mineral fertilizers when A. brasilense co-inoculation is performed in a cowpea crop. However, even in the case of an NPK basal fertilization, applied N rates should remain below 20 kg N ha−1 when co-inoculation with Bradyrhizobium sp. and A. brasilense is performed.

Original languageEnglish (US)
Article number1847
JournalPlants
Volume11
Issue number14
DOIs
StatePublished - Jul 2022

Bibliographical note

Funding Information:
This research was funded by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), grant number 2018/08485-7 and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, award number 312359/2017-9).

Publisher Copyright:
© 2022 by the authors.

Keywords

  • Vigna unguiculata (L.) Walp
  • biological nitrogen fixation
  • nitrogen fractions
  • nitrogen-
  • plant growth rhizobacteria

PubMed: MeSH publication types

  • Journal Article

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