Self-assembling peptides and peptide derivatives bearing cell-binding ligands are increasingly being investigated as defined cell culture matrices and as scaffolds for regenerative medicine. In order to systematically refine such scaffolds to elicit specific desired cell behaviors, ligand display should ideally be achieved without inadvertently altering other physicochemical properties such as viscoelasticity. Moreover, for in vivo applications, self-assembled biomaterials must exhibit low immunogenicity. In the present study, multi-peptide co-assembling hydrogels based on the β-sheet fibrillizing peptide Q11 (QQKFQFQFEQQ) were designed such that they presented RGDS or IKVAV ligands on their fibril surfaces. In co-assemblies of the ligand-bearing peptides with Q11, ligand incorporation levels capable of influencing the attachment, spreading, morphology, and growth of human umbilical vein endothelial cells (HUVECs) did not significantly alter the materials' fibrillization, β-turn secondary structure, or stiffness. RGDS-Q11 specifically increased HUVEC attachment, spreading, and growth when co-assembled into Q11 gels, whereas IKVAV-Q11 exerted a more subtle influence on attachment and morphology. Additionally, Q11 and RGDS-Q11 were minimally immunogenic in mice, making Q11-based biomaterials attractive candidates for further investigation as defined, modular extracellular matrices for applications in vitro and in vivo.
|Original language||English (US)|
|Number of pages||11|
|State||Published - Apr 2009|
Bibliographical noteFunding Information:
This research was supported in part by The American Heart Association (grant no. 0665218B), the National Science Foundation (grant no. CHE-0802286), and the National Institutes of Health (NIBIB, grant no. EB007335). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Biomedical Imaging and BioEngineering or the National Institutes of Health. We thank Karl Matlin and Jose Moyano for helpful discussions. TEM and CD were performed at the University of Chicago Electron Microscopy Facility and Biophysics Core Facility, respectively. We thank Dr. Dale Schaefer for access to the rheometer.
- 3-D culture
- Biomaterial immunogenicity
- Biomimetic material
- Regenerative medicine