Clusterin induced by N,N'-Dinitrosopiperazine is involved in nasopharyngeal carcinoma metastasis

Yuejin Li, Jinping Lu, Shan Zhou, Weiwei Wang, Gongjun Tan, Zhenlin Zhang, Zigang Dong, Tiebang Kang, Faqing Tang

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Nasopharyngeal carcinoma (NPC) has a high metastatic clinicopathological feature. As a carcinogen factor, N,N'-Dinitrosopiperazine (DNP) is involved in NPC metastasis, but its precise mechanism has not been fully elucidated. Herein, we showed that DNP promotes NPC metastasis through up-regulating anterior clusterin (CLU). DNP was found to increase CLU, matrix metalloproteinases (MMP) 9 and vascular endothelial growth factor (VEGF) expression and activity, further DNP-increased MMP-9 and VEGF expression was through up-regulating CLU. We also found that DNP increased the binding of CLU with MMP-9 or VEGF. DNP induced the motility and invasion of NPC cell, which was inhibited by siRNA-CLU. The clinical investigation showed that CLU, MMP-9 and VEGF were positively correlated with the tumor-node -metastasis (TNM) classification. These results indicate that DNP may promote NPC tumor metastasis through up-regulating CLU, MMP-9 and VEGF expression. Therefore, DNP-increased CLU expression may be an important factor of NPC-high metastasis, and CLU may serve as a biomarker for NPC metastasis.

Original languageEnglish (US)
Pages (from-to)5548-5563
Number of pages16
JournalOncotarget
Volume7
Issue number5
DOIs
StatePublished - 2016

Bibliographical note

Funding Information:
This work was supported in part by the National Natural Science Foundation of China (81372282, 81071718, 81402368, 81402265, 81502346), Program for New Century Excellent Talents in University, NCET (NCET-06-0685), Guangdong Natural Science Foundation (S2013010013360).

Keywords

  • Clusterin
  • Metastasis
  • N,N'-Dinitrosopiperazine
  • Nasopharyngeal carcinoma

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