Clostridium Species as Metallic Copper-Forming Bacteria in Soil under Reducing Conditions

Anke F. Hofacker; Sebastian Behrens; Andreas Voegelin; Ralf Kaegi; Tina Lösekann-Behrens; Andreas Kappler; Ruben Kretzschmar

doi:10.1080/01490451.2014.933287

Clostridium Species as Metallic Copper-Forming Bacteria in Soil under Reducing Conditions

Anke F. Hofacker, Sebastian Behrens, Andreas Voegelin, Ralf Kaegi, Tina Lösekann-Behrens, Andreas Kappler, Ruben Kretzschmar

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Recent studies have reported the formation of Cu⁰ nanoparticles (CuNP) by suspended bacteria in pore water of periodically flooded soils, but the bacteria have not yet been identified. The aim of this study was to identify the CuNP-forming bacteria and to determine the location of CuNP formation relative to the bacterial cell surface. Electron microscopy revealed that the bacteria were rod-shaped spore formers and suggested that CuNP were formed in the periplasm. Combined results from denaturing gradient gel electrophoresis, 16S rRNA gene clone libraries, and classic microbiological cultivation techniques provided strong evidence for a Clostridium sp. strain as the CuNP-forming bacteria. Clostridia are well-adapted to frequent flooding and drying due to their ability to form spores and may play an important role in Cu cycling and metallic Cu formation in redox-dynamic environments.

Original language	English (US)
Pages (from-to)	130-139
Number of pages	10
Journal	Geomicrobiology Journal
Volume	32
Issue number	2
DOIs	https://doi.org/10.1080/01490451.2014.933287
State	Published - Feb 7 2015

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Publisher Copyright:
© 2015, Copyright © Taylor & Francis Group, LLC.

Keywords

Clostridia
biomineralization
copA
copper
metals
molecular microbial ecology
nanoparticles
redox
soil
spore forming bacteria
wetland

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abstract = "Recent studies have reported the formation of Cu0 nanoparticles (CuNP) by suspended bacteria in pore water of periodically flooded soils, but the bacteria have not yet been identified. The aim of this study was to identify the CuNP-forming bacteria and to determine the location of CuNP formation relative to the bacterial cell surface. Electron microscopy revealed that the bacteria were rod-shaped spore formers and suggested that CuNP were formed in the periplasm. Combined results from denaturing gradient gel electrophoresis, 16S rRNA gene clone libraries, and classic microbiological cultivation techniques provided strong evidence for a Clostridium sp. strain as the CuNP-forming bacteria. Clostridia are well-adapted to frequent flooding and drying due to their ability to form spores and may play an important role in Cu cycling and metallic Cu formation in redox-dynamic environments.",

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AU - Hofacker, Anke F.

AU - Behrens, Sebastian

AU - Voegelin, Andreas

AU - Kaegi, Ralf

AU - Lösekann-Behrens, Tina

AU - Kappler, Andreas

AU - Kretzschmar, Ruben

PY - 2015/2/7

Y1 - 2015/2/7

N2 - Recent studies have reported the formation of Cu0 nanoparticles (CuNP) by suspended bacteria in pore water of periodically flooded soils, but the bacteria have not yet been identified. The aim of this study was to identify the CuNP-forming bacteria and to determine the location of CuNP formation relative to the bacterial cell surface. Electron microscopy revealed that the bacteria were rod-shaped spore formers and suggested that CuNP were formed in the periplasm. Combined results from denaturing gradient gel electrophoresis, 16S rRNA gene clone libraries, and classic microbiological cultivation techniques provided strong evidence for a Clostridium sp. strain as the CuNP-forming bacteria. Clostridia are well-adapted to frequent flooding and drying due to their ability to form spores and may play an important role in Cu cycling and metallic Cu formation in redox-dynamic environments.

AB - Recent studies have reported the formation of Cu0 nanoparticles (CuNP) by suspended bacteria in pore water of periodically flooded soils, but the bacteria have not yet been identified. The aim of this study was to identify the CuNP-forming bacteria and to determine the location of CuNP formation relative to the bacterial cell surface. Electron microscopy revealed that the bacteria were rod-shaped spore formers and suggested that CuNP were formed in the periplasm. Combined results from denaturing gradient gel electrophoresis, 16S rRNA gene clone libraries, and classic microbiological cultivation techniques provided strong evidence for a Clostridium sp. strain as the CuNP-forming bacteria. Clostridia are well-adapted to frequent flooding and drying due to their ability to form spores and may play an important role in Cu cycling and metallic Cu formation in redox-dynamic environments.

KW - Clostridia

KW - biomineralization

KW - copA

KW - copper

KW - metals

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Clostridium Species as Metallic Copper-Forming Bacteria in Soil under Reducing Conditions

Abstract

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