Cloning, sequencing, and expression of the pyruvate carboxylase gene in Lactococcus lactis subsp. lactis C2

H. Wang, D. J. O'Sullivan, K. A. Baldwin, L. L. McKay

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Abstract

A functional pyc gene was isolated from Lactococcus lactis subsp, lactis C2 and was found to complement a Pyc defect in L. lactis KB4. The deduced lactococcal Pyc protein was highly homologous to Pyc sequences of other bacteria. The pyc gene was also detected in Lactococcus lactis subsp. cremoris and L. lactis subsp. lactis bv. diacetylactis strains.

Original languageEnglish (US)
Pages (from-to)1223-1227
Number of pages5
JournalApplied and environmental microbiology
Volume66
Issue number3
DOIs
StatePublished - Mar 2000

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