Cloning, sequence, and tissue distribution of a rabbit renal Na+/H+ exchanger transcript

Friedhelm Hildebrandt, John H. Pizzonia, Robert F. Reilly, Nancy A. Rebouças, Claude Sardet, Jacques Pouysségur, Carolyn W. Slayman, Peter S. Aronson, Peter Igarashi

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27 Scopus citations

Abstract

Rabbit kidney expresses a transcript that is similar to the human growth-factor-activatable Na+/H+ exchanger. PCR and library screening were used to clone overlapping 2.5 kb, 1.4 kb, and 1.8 kb cDNAs that together contain the entire coding region (2448 bp) and 5′ untranslated region (726 bp) and part of the 3′ untranslated region (128 bp) of a rabbit renal Na+/H+ exchanger transcript. The nucleotide and inferred amino acid sequences are highly conserved between rabbit and human (88% nucleotide identity, 95% amino acid identity). In rabbit, the transcript is expressed in both epithelial and non-epithelial tissues, with highes expression in stomach, brain, kidney, lung and ileum, and minimal expression in liver and skeletal muscle.

Original languageEnglish (US)
Pages (from-to)105-108
Number of pages4
JournalBBA - Gene Structure and Expression
Volume1129
Issue number1
DOIs
StatePublished - Dec 2 1991

Bibliographical note

Funding Information:
The technical assistance of Marianne Sadowski, Jen-nifer Elsemore, Thecla Abbiati, and Christine Shugrue is gratefully acknowledged. This work was supported by the Deutsche Forschungsgemeinschaft; American Heart Association Connecticut Affiliate, Inc.: Funda-~fio de Amparo h Pesquisa do Estado de Silo Paulo; and U.S. Public Health Service (grants DK-33793, DK-42921. AM-01423, DK-07259, and DK-01816).

Keywords

  • (Rabbit kidney)
  • Amino acid sequence
  • Polymerase chain reaction
  • Sodium-proton exchange
  • cDNA cloning

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