Abstract
A search for the presence of mariner-like elements in the Labeo rohita genome by polymerase chain reaction led to the amplification of a partial DNA sequence coding for a putative transmembrane domain of gonadotropin hormone receptor. The amplified DNA sequence shows a high degree of homology to the available turkey and human luteinizing and follicle stimulating hormone receptor coding sequences. This is the first report on cloning such sequences of piscine origin.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 41-45 |
| Number of pages | 5 |
| Journal | Journal of Biosciences |
| Volume | 25 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 2000 |
| Externally published | Yes |
Bibliographical note
Funding Information:We thank the Department of Biotechnology, University Grants Commission and Indian Council of Agricultural Research, New Delhi for the financial assistance. We gratefully acknowledge Dr Lalji Singh (Centre for Cellular and Molecular Biology, Hyderabad) for providing DNA sequencing facility. The computer facility provided by the Bioinformatics Centre (Madurai) is acknowledged. Our sincere thanks to Dr M L Dufau (National Institute of Health, Maryland, USA) for the timely help.
Keywords
- Glycoprotein hormone receptor
- Gonadotropin receptor
- Labeo rohita
- Luteinizing hormone receptor
- Mariner transposon
- PCR cloning