Cloning of genes involved in the production of bacteriocin in Cicer - Rhizobium PR 2109a

J. Nirmala, Y. D. Gaur, P. K. Lawrence

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The molecular basis of bacteriocin production by a Cicer - Rhizobium strain PR2109a was studied. The bacterial strain showed in vitro growth inhibition of non-bacteriocin producing strain of Cicer - Rhizobium PR2005b. Tn5 mutagenesis of the wild-type strain helped in the isolation of the bacteriocin-defective mutant JN365. A genomic library of the wild-type strain was constructed in the cosmid vector pLAFR1 and maintained in Escherchia coli background. Complementation analysis with the cosmid library resulted in the isolation of a cosmid clone which complemented the defective character in the mutant JN365. The size of the complementary DNA fragment was found to be 23 kb.

Original languageEnglish (US)
Article number324847
Pages (from-to)47-49
Number of pages3
JournalWorld Journal of Microbiology and Biotechnology
Volume17
Issue number1
DOIs
StatePublished - Jan 1 2001

Keywords

  • Bacteriocin
  • Cicer - Rhizobium
  • Competitiveness

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