Cloning, expression and chromosomal localization of a human testis 6- phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene

A. Manzano; J. X. Pérez; M. Nadal; X. Estivill; A. Lange; R. Bartrons

doi:10.1016/S0378-1119(99)00037-2

Cloning, expression and chromosomal localization of a human testis 6- phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene

A. Manzano
, J. X. Pérez
, M. Nadal
, X. Estivill
, A. Lange
, R. Bartrons

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

6-Phosphofructo-2-kinase/fructose 2,6-bisphosphatase (PFK-2/FBPase-2) is a bifunctional enzyme responsible for the synthesis and breakdown of Fru- 2,6-P₂, a key metabolite in the regulation of glycolysis. Several genes encode distinct PFK-2/FBPase-2 isozymes that differ in their tissue distribution and enzyme regulation. In this paper, we present the isolation of a cDNA from a human testis cDNA library that encodes a PFK-2/FBPase-2 isozyme. Sequencing data show an open reading frame of 1407 nucleotides that codifies for a protein of 469 amino acids. This has a calculated molecular weight of 54 kDa and 97% similarity with rat testis PFK-2/FBPase-2, with complete conservation of the amino acid residues involved in the catalytic mechanism. Fluorescence in-situ hybridization (FISH) localized testis PFK- 2/FBPase-2 gene (PFKFB4) in human chromosome 3 at bands p21-p22. A Northern blot analysis of different rat tissues showed the presence of a 2.4-kb mRNA expressed specifically in testis. In mammalian COS-1 cells, the human testis cDNA drives expression of an isozyme with a molecular weight of 55 kDa. This isozyme shows clear PFK-2 activity. Taken together, these results provide evidence for a new PFK-2/FBPase-2 gene coding for a human testis isozyme.

Original language	English (US)
Pages (from-to)	83-89
Number of pages	7
Journal	Gene
Volume	229
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-1119(99)00037-2
State	Published - Mar 18 1999
Externally published	Yes

Bibliographical note

Funding Information:
This study was supported by FIS 95/0286 and DGICYT (PM97/0114) and Generalitat de Catalunya (1997SGR00074). A.M. received a research fellowship from Ministerio de Educación y Ciencia.

Keywords

6-phosphofructo-2-kinase/fructose 2,6- bisphosphatase
Gene
Glycolysis
Testis

Publisher link

10.1016/S0378-1119(99)00037-2

Find It

Find It at U of M Libraries

Cite this

@article{9359aacc584b4bf5bdf709f67c42ad1a,

title = "Cloning, expression and chromosomal localization of a human testis 6- phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene",

abstract = "6-Phosphofructo-2-kinase/fructose 2,6-bisphosphatase (PFK-2/FBPase-2) is a bifunctional enzyme responsible for the synthesis and breakdown of Fru- 2,6-P2, a key metabolite in the regulation of glycolysis. Several genes encode distinct PFK-2/FBPase-2 isozymes that differ in their tissue distribution and enzyme regulation. In this paper, we present the isolation of a cDNA from a human testis cDNA library that encodes a PFK-2/FBPase-2 isozyme. Sequencing data show an open reading frame of 1407 nucleotides that codifies for a protein of 469 amino acids. This has a calculated molecular weight of 54 kDa and 97\% similarity with rat testis PFK-2/FBPase-2, with complete conservation of the amino acid residues involved in the catalytic mechanism. Fluorescence in-situ hybridization (FISH) localized testis PFK- 2/FBPase-2 gene (PFKFB4) in human chromosome 3 at bands p21-p22. A Northern blot analysis of different rat tissues showed the presence of a 2.4-kb mRNA expressed specifically in testis. In mammalian COS-1 cells, the human testis cDNA drives expression of an isozyme with a molecular weight of 55 kDa. This isozyme shows clear PFK-2 activity. Taken together, these results provide evidence for a new PFK-2/FBPase-2 gene coding for a human testis isozyme.",

keywords = "6-phosphofructo-2-kinase/fructose 2,6- bisphosphatase, Gene, Glycolysis, Testis",

author = "A. Manzano and P{\'e}rez, \{J. X.\} and M. Nadal and X. Estivill and A. Lange and R. Bartrons",

note = "Funding Information: This study was supported by FIS 95/0286 and DGICYT (PM97/0114) and Generalitat de Catalunya (1997SGR00074). A.M. received a research fellowship from Ministerio de Educaci{\'o}n y Ciencia.",

year = "1999",

month = mar,

day = "18",

doi = "10.1016/S0378-1119(99)00037-2",

language = "English (US)",

volume = "229",

pages = "83--89",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier B.V.",

number = "1-2",

}

TY - JOUR

T1 - Cloning, expression and chromosomal localization of a human testis 6- phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene

AU - Manzano, A.

AU - Pérez, J. X.

AU - Nadal, M.

AU - Estivill, X.

AU - Lange, A.

AU - Bartrons, R.

N1 - Funding Information: This study was supported by FIS 95/0286 and DGICYT (PM97/0114) and Generalitat de Catalunya (1997SGR00074). A.M. received a research fellowship from Ministerio de Educación y Ciencia.

PY - 1999/3/18

Y1 - 1999/3/18

N2 - 6-Phosphofructo-2-kinase/fructose 2,6-bisphosphatase (PFK-2/FBPase-2) is a bifunctional enzyme responsible for the synthesis and breakdown of Fru- 2,6-P2, a key metabolite in the regulation of glycolysis. Several genes encode distinct PFK-2/FBPase-2 isozymes that differ in their tissue distribution and enzyme regulation. In this paper, we present the isolation of a cDNA from a human testis cDNA library that encodes a PFK-2/FBPase-2 isozyme. Sequencing data show an open reading frame of 1407 nucleotides that codifies for a protein of 469 amino acids. This has a calculated molecular weight of 54 kDa and 97% similarity with rat testis PFK-2/FBPase-2, with complete conservation of the amino acid residues involved in the catalytic mechanism. Fluorescence in-situ hybridization (FISH) localized testis PFK- 2/FBPase-2 gene (PFKFB4) in human chromosome 3 at bands p21-p22. A Northern blot analysis of different rat tissues showed the presence of a 2.4-kb mRNA expressed specifically in testis. In mammalian COS-1 cells, the human testis cDNA drives expression of an isozyme with a molecular weight of 55 kDa. This isozyme shows clear PFK-2 activity. Taken together, these results provide evidence for a new PFK-2/FBPase-2 gene coding for a human testis isozyme.

AB - 6-Phosphofructo-2-kinase/fructose 2,6-bisphosphatase (PFK-2/FBPase-2) is a bifunctional enzyme responsible for the synthesis and breakdown of Fru- 2,6-P2, a key metabolite in the regulation of glycolysis. Several genes encode distinct PFK-2/FBPase-2 isozymes that differ in their tissue distribution and enzyme regulation. In this paper, we present the isolation of a cDNA from a human testis cDNA library that encodes a PFK-2/FBPase-2 isozyme. Sequencing data show an open reading frame of 1407 nucleotides that codifies for a protein of 469 amino acids. This has a calculated molecular weight of 54 kDa and 97% similarity with rat testis PFK-2/FBPase-2, with complete conservation of the amino acid residues involved in the catalytic mechanism. Fluorescence in-situ hybridization (FISH) localized testis PFK- 2/FBPase-2 gene (PFKFB4) in human chromosome 3 at bands p21-p22. A Northern blot analysis of different rat tissues showed the presence of a 2.4-kb mRNA expressed specifically in testis. In mammalian COS-1 cells, the human testis cDNA drives expression of an isozyme with a molecular weight of 55 kDa. This isozyme shows clear PFK-2 activity. Taken together, these results provide evidence for a new PFK-2/FBPase-2 gene coding for a human testis isozyme.

KW - 6-phosphofructo-2-kinase/fructose 2,6- bisphosphatase

KW - Gene

KW - Glycolysis

KW - Testis

UR - https://www.scopus.com/pages/publications/0344923803

UR - https://www.scopus.com/pages/publications/0344923803#tab=citedBy

U2 - 10.1016/S0378-1119(99)00037-2

DO - 10.1016/S0378-1119(99)00037-2

M3 - Article

C2 - 10095107

AN - SCOPUS:0344923803

SN - 0378-1119

VL - 229

SP - 83

EP - 89

JO - Gene

JF - Gene

IS - 1-2

ER -

Cloning, expression and chromosomal localization of a human testis 6- phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene

Abstract

Bibliographical note

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this