Cloning and Regulation of Schizosaccharomyces pombe Gene Encoding Ribosomal Protein L11

Hong Gyum Kim, Jin Joo Lee, Eun Hee Park, Jae Hoon Sa, Ki Sup Ahn, Chang Jin Lim

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7 Scopus citations

Abstract

The cDNA encoding ribosomal protein was identified from a cDNA library of Schizosaccharomyces pombe. The nucleotide sequence of the 548 bp cDNA clone reveals an open reading frame, which encodes a putative protein of 166 amino acids with a molecular mass of 18.3 kDa. The amino acid sequence of the S. pombe L11 protein is highly homologous with those of rat and fruit, while it is clearly less similar to those of prokaryotic counterparts. The 1,044 bp upstream sequence, and the region encoding N-terminal 7 amino acids of the genomic DNA were fused into the promoterless β-galactosidase gene of the shuttle vector YEp357 in order to generate the fusion plasmid pHY L11. Synthesis of β-galactosidase from the fusion plasmid varied according to the growth curve. It decreased significantly in the growth-arrested yeast cells that were treated with aluminum chloride and mercuric chloride. However, it was enhanced by treatments with cadmium chloride (2.5 μM), zinc chloride (2.5 μM), and hydrogen peroxide (0.5 mM). This indicates that the expression of the L11 gene could be induced by oxidative stress.

Original languageEnglish (US)
Pages (from-to)379-384
Number of pages6
JournalJournal of Biochemistry and Molecular Biology
Volume34
Issue number4
StatePublished - Jul 31 2001

Keywords

  • β-Galactosidase fusion
  • cDNA
  • Fission yeast
  • L11
  • Regulation
  • Ribosomal protein
  • Schizosaccharomyces pombe

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    Kim, H. G., Lee, J. J., Park, E. H., Sa, J. H., Ahn, K. S., & Lim, C. J. (2001). Cloning and Regulation of Schizosaccharomyces pombe Gene Encoding Ribosomal Protein L11. Journal of Biochemistry and Molecular Biology, 34(4), 379-384.