Cloning and expression pattern of Xenopus prx-1 (Xprx-1) during embryonic development

Shuji Takahashi, Takaaki Uochi, Yasuhiko Kawakami, Tsutomu Nohno, Chika Yokota, Kei Kinoshita, Makoto Asashima

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Homeobox genes are expressed both temporally and spatially during vertebrate development, and regulate the tissue-specific expression of other genes. A Xenopus paired-related homeobox-1 (Xprx-1) cDNA was cloned. Xprx-1 had a paired-related homeodomain, but did not contain a paired-box. The sequence of Xprx-1 had a high level of homology with K-2(mouse) and Prx-1 (chicken), thus Xprx-1 is assumed to be the Xenopus homolog of these genes. Xprx-1 transcripts were maternally restricted, in Xenopus embryos, and a decrease in the late blastula stage was followed by an increase in zygotic transcripts after gastrulation. The transcripts were localized to the animal hemisphere of the late blastula and were concentrated in the branchial arches of the tail-bud stage embryo. In animal cap experiments, Activin A dose- dependently induced Xprx-1 gene expression. These results suggest that Xprx- 1 plays a role in early Xenopus development similar to other species.

Original languageEnglish (US)
Pages (from-to)97-104
Number of pages8
JournalDevelopment Growth and Differentiation
Volume40
Issue number1
DOIs
StatePublished - 1998

Keywords

  • Activin
  • Paired-related homeobox genes
  • Prx-1
  • Xenopus laevis

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