Cloning and complete sequence of an HLA-A2 gene: Analysis of two HLA-A alleles at the nucleotide level

B. H. Koller, H. T. Orr

Research output: Contribution to journalArticle

201 Citations (Scopus)

Abstract

The gene for the HLA-A2 antigen has been cloned from the human lymphoblastoic cell line 721. Comparison of this sequence with the published sequence for HLA-A3 permits the examination of two alleles at the extremely polymorphic HLA-A locus. A high degree of sequence conservation was seen in both coding and noncodong DNA, 97.2% and 94.5%, respectively. Interestingly, the 3' untranslated region was the most conserved, with 99.5% homology. The polymorphism of the HLA-A antigens results from a high proportion of amino acid substitutions relative to the total nucleotide changes in exons 2 and 3. Unlike the clustered differences seen in this region on comparison of two H-2K alleles of mouse, nucleotide substitutions between the HLA-A2 and A3 alleles are evenly distributed. Substitutions at silent sites and within introns were used to calculate an intra-allelic divergence time of at least 10 to 15 million years for these two HLA-A alleles.

Original languageEnglish (US)
Pages (from-to)2727-2733
Number of pages7
JournalJournal of Immunology
Volume134
Issue number4
StatePublished - Jan 1 1985

Fingerprint

HLA-A2 Antigen
HLA-A Antigens
Organism Cloning
Nucleotides
HLA-A3 Antigen
Alleles
Genes
3' Untranslated Regions
Amino Acid Substitution
Introns
Exons
Cell Line
DNA

Cite this

Cloning and complete sequence of an HLA-A2 gene : Analysis of two HLA-A alleles at the nucleotide level. / Koller, B. H.; Orr, H. T.

In: Journal of Immunology, Vol. 134, No. 4, 01.01.1985, p. 2727-2733.

Research output: Contribution to journalArticle

@article{921b0697afe345029e7f42223a181c8a,
title = "Cloning and complete sequence of an HLA-A2 gene: Analysis of two HLA-A alleles at the nucleotide level",
abstract = "The gene for the HLA-A2 antigen has been cloned from the human lymphoblastoic cell line 721. Comparison of this sequence with the published sequence for HLA-A3 permits the examination of two alleles at the extremely polymorphic HLA-A locus. A high degree of sequence conservation was seen in both coding and noncodong DNA, 97.2{\%} and 94.5{\%}, respectively. Interestingly, the 3' untranslated region was the most conserved, with 99.5{\%} homology. The polymorphism of the HLA-A antigens results from a high proportion of amino acid substitutions relative to the total nucleotide changes in exons 2 and 3. Unlike the clustered differences seen in this region on comparison of two H-2K alleles of mouse, nucleotide substitutions between the HLA-A2 and A3 alleles are evenly distributed. Substitutions at silent sites and within introns were used to calculate an intra-allelic divergence time of at least 10 to 15 million years for these two HLA-A alleles.",
author = "Koller, {B. H.} and Orr, {H. T.}",
year = "1985",
month = "1",
day = "1",
language = "English (US)",
volume = "134",
pages = "2727--2733",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "4",

}

TY - JOUR

T1 - Cloning and complete sequence of an HLA-A2 gene

T2 - Analysis of two HLA-A alleles at the nucleotide level

AU - Koller, B. H.

AU - Orr, H. T.

PY - 1985/1/1

Y1 - 1985/1/1

N2 - The gene for the HLA-A2 antigen has been cloned from the human lymphoblastoic cell line 721. Comparison of this sequence with the published sequence for HLA-A3 permits the examination of two alleles at the extremely polymorphic HLA-A locus. A high degree of sequence conservation was seen in both coding and noncodong DNA, 97.2% and 94.5%, respectively. Interestingly, the 3' untranslated region was the most conserved, with 99.5% homology. The polymorphism of the HLA-A antigens results from a high proportion of amino acid substitutions relative to the total nucleotide changes in exons 2 and 3. Unlike the clustered differences seen in this region on comparison of two H-2K alleles of mouse, nucleotide substitutions between the HLA-A2 and A3 alleles are evenly distributed. Substitutions at silent sites and within introns were used to calculate an intra-allelic divergence time of at least 10 to 15 million years for these two HLA-A alleles.

AB - The gene for the HLA-A2 antigen has been cloned from the human lymphoblastoic cell line 721. Comparison of this sequence with the published sequence for HLA-A3 permits the examination of two alleles at the extremely polymorphic HLA-A locus. A high degree of sequence conservation was seen in both coding and noncodong DNA, 97.2% and 94.5%, respectively. Interestingly, the 3' untranslated region was the most conserved, with 99.5% homology. The polymorphism of the HLA-A antigens results from a high proportion of amino acid substitutions relative to the total nucleotide changes in exons 2 and 3. Unlike the clustered differences seen in this region on comparison of two H-2K alleles of mouse, nucleotide substitutions between the HLA-A2 and A3 alleles are evenly distributed. Substitutions at silent sites and within introns were used to calculate an intra-allelic divergence time of at least 10 to 15 million years for these two HLA-A alleles.

UR - http://www.scopus.com/inward/record.url?scp=0021919448&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021919448&partnerID=8YFLogxK

M3 - Article

C2 - 2982951

AN - SCOPUS:0021919448

VL - 134

SP - 2727

EP - 2733

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 4

ER -