Cloning and characterization of wnt-4 and wnt-11 cDNAs from chick embryo

Nobuya Tanda, Yasuhiko Kawakami, Taiichi Saito, Sumihare Noji, Tsutomu Nohno

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

We have isolated two members of the Wnt gene family, Wnt-4 and Wnt-11, from chick embryo cDNA library, and determined the entire coding sequences. The Wnt-4 and Wnt-11 genes encode secretory proteins composed of 351 and 354 amino acids, respectively, both having 24 Cys residues conserved among other Wnt family members. Alignment of the deduced amino acid sequences reveals that chicken Wnt-4 and Wnt-11 are most similar to Xenopus Wnt-4 and mouse Wnt-11, respectively. Northern blot analysis indicates the Wnt-4 expression at 1.5 kilobase and the Wnt-11 expression at 2.0 kilobase in the chick embryo.

Original languageEnglish (US)
Pages (from-to)277-281
Number of pages5
JournalMitochondrial DNA
Volume5
Issue number5
DOIs
StatePublished - Jan 1 1995

Keywords

  • CDNA
  • Chick embryo
  • Growth factor
  • Wnt-11
  • Wnt-4

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