We have isolated two members of the Wnt gene family, Wnt-4 and Wnt-11, from chick embryo cDNA library, and determined the entire coding sequences. The Wnt-4 and Wnt-11 genes encode secretory proteins composed of 351 and 354 amino acids, respectively, both having 24 Cys residues conserved among other Wnt family members. Alignment of the deduced amino acid sequences reveals that chicken Wnt-4 and Wnt-11 are most similar to Xenopus Wnt-4 and mouse Wnt-11, respectively. Northern blot analysis indicates the Wnt-4 expression at 1.5 kilobase and the Wnt-11 expression at 2.0 kilobase in the chick embryo.
Bibliographical noteFunding Information:
This work was supported iii part by Grants-in-Aids from the Japanese Ministry of Education, Science and Culture, and by a Research Project Grant from Kawasaki Medical School.
- Chick embryo
- Growth factor