Cloning and characterization of aerobactin biosynthesis genes of the biological control agent Enterobacter cloacae

J. E. Loper, C. A. Ishimaru, S. R. Carnegie, A. Vanavichit

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Five strains of Enterobacter cloacae that are biological control agents of Pythium damping-off diseases produced the hydroxamate siderophore aerobactin under iron-limiting conditions. Genes determining aerobactin biosynthesis of the biocontrol strain E. cloacae EcCT-501 were localized to a 12.3-kb region, which conferred aerobactin production to Escherichia coli DH5α. The aerobactin biosynthesis genes of E. cloacae hybridized to those of the pCoIV- K30 plasmid of E. coli, but restriction patterns of the aerobactin regions of pCoIV-K30 and E. cloacae differed. A derivative strain with a deletion in the aerobactin biosynthesis locus was as effective as strain EcCT-501 in biological control of Pythium damping-off of cucumber. Thus, aerobactin production did not contribute significantly to the biological control activity of EcCT-501 under the conditions of this study.

Original languageEnglish (US)
Pages (from-to)4189-4197
Number of pages9
JournalApplied and environmental microbiology
Volume59
Issue number12
DOIs
StatePublished - 1993

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