Cloning and characterization of aerobactin biosynthesis genes of the biological control agent Enterobacter cloacae

J. E. Loper; C. A. Ishimaru; S. R. Carnegie; A. Vanavichit

doi:10.1128/aem.59.12.4189-4197.1993

Cloning and characterization of aerobactin biosynthesis genes of the biological control agent Enterobacter cloacae

J. E. Loper, C. A. Ishimaru, S. R. Carnegie, A. Vanavichit

Plant Pathology

Research output: Contribution to journal › Article › peer-review

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Abstract

Five strains of Enterobacter cloacae that are biological control agents of Pythium damping-off diseases produced the hydroxamate siderophore aerobactin under iron-limiting conditions. Genes determining aerobactin biosynthesis of the biocontrol strain E. cloacae EcCT-501 were localized to a 12.3-kb region, which conferred aerobactin production to Escherichia coli DH5α. The aerobactin biosynthesis genes of E. cloacae hybridized to those of the pCoIV- K30 plasmid of E. coli, but restriction patterns of the aerobactin regions of pCoIV-K30 and E. cloacae differed. A derivative strain with a deletion in the aerobactin biosynthesis locus was as effective as strain EcCT-501 in biological control of Pythium damping-off of cucumber. Thus, aerobactin production did not contribute significantly to the biological control activity of EcCT-501 under the conditions of this study.

Original language	English (US)
Pages (from-to)	4189-4197
Number of pages	9
Journal	Applied and environmental microbiology
Volume	59
Issue number	12
DOIs	https://doi.org/10.1128/aem.59.12.4189-4197.1993
State	Published - 1993

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title = "Cloning and characterization of aerobactin biosynthesis genes of the biological control agent Enterobacter cloacae",

abstract = "Five strains of Enterobacter cloacae that are biological control agents of Pythium damping-off diseases produced the hydroxamate siderophore aerobactin under iron-limiting conditions. Genes determining aerobactin biosynthesis of the biocontrol strain E. cloacae EcCT-501 were localized to a 12.3-kb region, which conferred aerobactin production to Escherichia coli DH5α. The aerobactin biosynthesis genes of E. cloacae hybridized to those of the pCoIV- K30 plasmid of E. coli, but restriction patterns of the aerobactin regions of pCoIV-K30 and E. cloacae differed. A derivative strain with a deletion in the aerobactin biosynthesis locus was as effective as strain EcCT-501 in biological control of Pythium damping-off of cucumber. Thus, aerobactin production did not contribute significantly to the biological control activity of EcCT-501 under the conditions of this study.",

author = "Loper, {J. E.} and Ishimaru, {C. A.} and Carnegie, {S. R.} and A. Vanavichit",

year = "1993",

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T1 - Cloning and characterization of aerobactin biosynthesis genes of the biological control agent Enterobacter cloacae

AU - Loper, J. E.

AU - Ishimaru, C. A.

AU - Carnegie, S. R.

AU - Vanavichit, A.

PY - 1993

Y1 - 1993

N2 - Five strains of Enterobacter cloacae that are biological control agents of Pythium damping-off diseases produced the hydroxamate siderophore aerobactin under iron-limiting conditions. Genes determining aerobactin biosynthesis of the biocontrol strain E. cloacae EcCT-501 were localized to a 12.3-kb region, which conferred aerobactin production to Escherichia coli DH5α. The aerobactin biosynthesis genes of E. cloacae hybridized to those of the pCoIV- K30 plasmid of E. coli, but restriction patterns of the aerobactin regions of pCoIV-K30 and E. cloacae differed. A derivative strain with a deletion in the aerobactin biosynthesis locus was as effective as strain EcCT-501 in biological control of Pythium damping-off of cucumber. Thus, aerobactin production did not contribute significantly to the biological control activity of EcCT-501 under the conditions of this study.

AB - Five strains of Enterobacter cloacae that are biological control agents of Pythium damping-off diseases produced the hydroxamate siderophore aerobactin under iron-limiting conditions. Genes determining aerobactin biosynthesis of the biocontrol strain E. cloacae EcCT-501 were localized to a 12.3-kb region, which conferred aerobactin production to Escherichia coli DH5α. The aerobactin biosynthesis genes of E. cloacae hybridized to those of the pCoIV- K30 plasmid of E. coli, but restriction patterns of the aerobactin regions of pCoIV-K30 and E. cloacae differed. A derivative strain with a deletion in the aerobactin biosynthesis locus was as effective as strain EcCT-501 in biological control of Pythium damping-off of cucumber. Thus, aerobactin production did not contribute significantly to the biological control activity of EcCT-501 under the conditions of this study.

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JO - Applied and environmental microbiology

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ER -

Cloning and characterization of aerobactin biosynthesis genes of the biological control agent Enterobacter cloacae

Abstract

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