Cloned transgenic calves produced from nonquiescent fetal fibroblasts

Jose B. Cibelli, Steve L. Stice, Paul J. Golueke, Jeff J. Kane, Joseph Jerry, Cathy Blackwell, F. Abel Ponce De León, James M. Robl

Research output: Contribution to journalArticlepeer-review

1092 Scopus citations

Abstract

An efficient system for genetic modification and large-scale cloning of cattle is of importance for agriculture, biotechnology, and human medicine. Here, actively dividing fetal fibroblasts were genetically modified with a marker gene, a clonal line was selected, and the cells were fused to enucleated mature oocytes. Out of 28 embryos transferred to 11 recipient cows, three healthy, identical, transgenic calves were generated. Furthermore, the life-span of near senescent fibroblasts could be extended by nuclear transfer, as indicated by population doublings in fibroblast lines derived from a 40-day-old fetal clone. With the ability to extend the life- span of these primary cultured cells, this system would be useful for inducing complex genetic modifications in cattle.

Original languageEnglish (US)
Pages (from-to)1256-1258
Number of pages3
JournalScience
Volume280
Issue number5367
DOIs
StatePublished - May 22 1998

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