Clonal and pathotypic analysis of archetypal Escherichia coli cystitis isolate NU14

James R Johnson; Scott J. Weissman; Adam L. Stell; Elena Trintchina; Daniel E. Dykhuizen; Evgeni V. Sokurenko

doi:10.1086/323891

Clonal and pathotypic analysis of archetypal Escherichia coli cystitis isolate NU14

James R Johnson
, Scott J. Weissman
, Adam L. Stell
, Elena Trintchina
, Daniel E. Dykhuizen
, Evgeni V. Sokurenko

Medicine - Veteran's Administration Medical Center

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

Escherichia coli NU14, a cystitis isolate used to study the pathogenesis of cystitis and to develop a FimH (type 1 fimbrial adhesin) vaccine, was assessed for extended virulence genotype, phylogenetic background, and FimH sequence and binding phenotype(s). NU14 exhibited the same virulence genotype and was derived from the same (meningitis- and cystitis-associated) subclone of E. coli O18:K1:H7 as the archetypal neonatal bacterial meningitis (NBM) isolate RS218. NU14 also displayed the same Ser62Ala FimH polymorphism as did NBM isolates RS218 and IHE3034 - conferring both collagen binding and a distinct monomannose binding capability (which characterizes uropathogenic but not commensal E. coli and dramatically increases adherence to uroepithelial cells). These findings establish that strain NU14 exhibits numerous urovirulence-associated traits and derives from the single most prevalent clonal group in acute cystitis. They provide further evidence of clonal and pathotypic similarities between cystitis and NBM isolates of E. coli O18:K1:H7.

Original language	English (US)
Pages (from-to)	1556-1565
Number of pages	10
Journal	Journal of Infectious Diseases
Volume	184
Issue number	12
DOIs	https://doi.org/10.1086/323891
State	Published - Dec 15 2001

Bibliographical note

Funding Information:
Financial support: Office of Research and Development, Medical Research Service, Department of Veterans Affairs (to J.R.J.); National Institutes of Health (grants DK-47504 to J.R.J., AI-45820 to E.V.S., GM-60731 to D.E.D., and 5T32 HD07233 [fellowship training] to S.J.W.); National Research Initiative competitive grants program/US Department of Agriculture (grant 00-35212-9408 to J.R.J.).

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title = "Clonal and pathotypic analysis of archetypal Escherichia coli cystitis isolate NU14",

abstract = "Escherichia coli NU14, a cystitis isolate used to study the pathogenesis of cystitis and to develop a FimH (type 1 fimbrial adhesin) vaccine, was assessed for extended virulence genotype, phylogenetic background, and FimH sequence and binding phenotype(s). NU14 exhibited the same virulence genotype and was derived from the same (meningitis- and cystitis-associated) subclone of E. coli O18:K1:H7 as the archetypal neonatal bacterial meningitis (NBM) isolate RS218. NU14 also displayed the same Ser62Ala FimH polymorphism as did NBM isolates RS218 and IHE3034 - conferring both collagen binding and a distinct monomannose binding capability (which characterizes uropathogenic but not commensal E. coli and dramatically increases adherence to uroepithelial cells). These findings establish that strain NU14 exhibits numerous urovirulence-associated traits and derives from the single most prevalent clonal group in acute cystitis. They provide further evidence of clonal and pathotypic similarities between cystitis and NBM isolates of E. coli O18:K1:H7.",

author = "Johnson, \{James R\} and Weissman, \{Scott J.\} and Stell, \{Adam L.\} and Elena Trintchina and Dykhuizen, \{Daniel E.\} and Sokurenko, \{Evgeni V.\}",

note = "Funding Information: Financial support: Office of Research and Development, Medical Research Service, Department of Veterans Affairs (to J.R.J.); National Institutes of Health (grants DK-47504 to J.R.J., AI-45820 to E.V.S., GM-60731 to D.E.D., and 5T32 HD07233 [fellowship training] to S.J.W.); National Research Initiative competitive grants program/US Department of Agriculture (grant 00-35212-9408 to J.R.J.).",

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AU - Dykhuizen, Daniel E.

AU - Sokurenko, Evgeni V.

N1 - Funding Information: Financial support: Office of Research and Development, Medical Research Service, Department of Veterans Affairs (to J.R.J.); National Institutes of Health (grants DK-47504 to J.R.J., AI-45820 to E.V.S., GM-60731 to D.E.D., and 5T32 HD07233 [fellowship training] to S.J.W.); National Research Initiative competitive grants program/US Department of Agriculture (grant 00-35212-9408 to J.R.J.).

PY - 2001/12/15

Y1 - 2001/12/15

N2 - Escherichia coli NU14, a cystitis isolate used to study the pathogenesis of cystitis and to develop a FimH (type 1 fimbrial adhesin) vaccine, was assessed for extended virulence genotype, phylogenetic background, and FimH sequence and binding phenotype(s). NU14 exhibited the same virulence genotype and was derived from the same (meningitis- and cystitis-associated) subclone of E. coli O18:K1:H7 as the archetypal neonatal bacterial meningitis (NBM) isolate RS218. NU14 also displayed the same Ser62Ala FimH polymorphism as did NBM isolates RS218 and IHE3034 - conferring both collagen binding and a distinct monomannose binding capability (which characterizes uropathogenic but not commensal E. coli and dramatically increases adherence to uroepithelial cells). These findings establish that strain NU14 exhibits numerous urovirulence-associated traits and derives from the single most prevalent clonal group in acute cystitis. They provide further evidence of clonal and pathotypic similarities between cystitis and NBM isolates of E. coli O18:K1:H7.

AB - Escherichia coli NU14, a cystitis isolate used to study the pathogenesis of cystitis and to develop a FimH (type 1 fimbrial adhesin) vaccine, was assessed for extended virulence genotype, phylogenetic background, and FimH sequence and binding phenotype(s). NU14 exhibited the same virulence genotype and was derived from the same (meningitis- and cystitis-associated) subclone of E. coli O18:K1:H7 as the archetypal neonatal bacterial meningitis (NBM) isolate RS218. NU14 also displayed the same Ser62Ala FimH polymorphism as did NBM isolates RS218 and IHE3034 - conferring both collagen binding and a distinct monomannose binding capability (which characterizes uropathogenic but not commensal E. coli and dramatically increases adherence to uroepithelial cells). These findings establish that strain NU14 exhibits numerous urovirulence-associated traits and derives from the single most prevalent clonal group in acute cystitis. They provide further evidence of clonal and pathotypic similarities between cystitis and NBM isolates of E. coli O18:K1:H7.

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Clonal and pathotypic analysis of archetypal Escherichia coli cystitis isolate NU14

Abstract

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