Clonal and pathotypic analysis of archetypal Escherichia coli cystitis isolate NU14

James R Johnson, Scott J. Weissman, Adam L. Stell, Elena Trintchina, Daniel E. Dykhuizen, Evgeni V. Sokurenko

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


Escherichia coli NU14, a cystitis isolate used to study the pathogenesis of cystitis and to develop a FimH (type 1 fimbrial adhesin) vaccine, was assessed for extended virulence genotype, phylogenetic background, and FimH sequence and binding phenotype(s). NU14 exhibited the same virulence genotype and was derived from the same (meningitis- and cystitis-associated) subclone of E. coli O18:K1:H7 as the archetypal neonatal bacterial meningitis (NBM) isolate RS218. NU14 also displayed the same Ser62Ala FimH polymorphism as did NBM isolates RS218 and IHE3034 - conferring both collagen binding and a distinct monomannose binding capability (which characterizes uropathogenic but not commensal E. coli and dramatically increases adherence to uroepithelial cells). These findings establish that strain NU14 exhibits numerous urovirulence-associated traits and derives from the single most prevalent clonal group in acute cystitis. They provide further evidence of clonal and pathotypic similarities between cystitis and NBM isolates of E. coli O18:K1:H7.

Original languageEnglish (US)
Pages (from-to)1556-1565
Number of pages10
JournalJournal of Infectious Diseases
Issue number12
StatePublished - Dec 15 2001

Bibliographical note

Funding Information:
Financial support: Office of Research and Development, Medical Research Service, Department of Veterans Affairs (to J.R.J.); National Institutes of Health (grants DK-47504 to J.R.J., AI-45820 to E.V.S., GM-60731 to D.E.D., and 5T32 HD07233 [fellowship training] to S.J.W.); National Research Initiative competitive grants program/US Department of Agriculture (grant 00-35212-9408 to J.R.J.).

Fingerprint Dive into the research topics of 'Clonal and pathotypic analysis of archetypal Escherichia coli cystitis isolate NU14'. Together they form a unique fingerprint.

Cite this