Clinicopathologic significance of the K-ras gene codon 12 point mutation in stomach cancer. An analysis of 140 cases

K H Lee, J S Lee, C Suh, S W Kim, S B Kim, J H Lee, M S Lee, M Y Park, H S Sun, S B Kim

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62 Scopus citations

Abstract

BACKGROUND: The frequency and clinicopathologic significance of the K-ras gene point mutation in stomach cancer remain to be defined.

METHODS: The authors investigated the frequency of K-ras codon 12 point mutations in stomach cancer using a sensitive polymerase chain reaction (PCR)-based method in 140 samples and correlated the findings with various clinicopathologic characteristics of the patients.

RESULTS: The overall frequency of K-ras codon 12 point mutations in stomach cancer was 7.9% (11/140). DNA sequencing of nine cases with K-ras codon 12 point mutations identified seven cases with a single-base substitution of GGT to AGT (glycine to serine) and two with single-base substitution of GGT to AGT (aspartic acid). Tumors located in the upper third of the stomach had a significantly higher frequency of K-ras codon 12 mutations (3/8, 37.5%) compared with tumors located in the middle (4/29, 13.8%) or lower (3/99, 3.0%) thirds of the stomach (P = 0.001). No significant difference was observed in the frequency of K-ras codon 12 mutations in terms of other various clinicopathologic characteristics including tumor DNA ploidy and S-phase fraction. After a median follow-up of 26 months, disease free and overall survival were not significantly different between patients with stomach cancer with or without K-ras codon 12 mutation. Among eight patients with stomach cancer located in the upper part of the stomach, none of the three patients with K-ras gene-mutated tumors died versus four of five with tumors without K-ras gene mutations (P = 0.064).

CONCLUSIONS: K-ras codon 12 point mutations are uncommon in stomach cancer (7.9%). There was significant correlation between K-ras mutations and vertical tumor location in the stomach, suggesting that different mechanisms may play a role in the pathogenesis of stomach cancer according to the location of tumors in the stomach.

Original languageEnglish (US)
Pages (from-to)2794-801
Number of pages8
JournalCancer
Volume75
Issue number12
DOIs
StatePublished - Jun 15 1995

Keywords

  • Adult
  • Aged
  • Base Sequence
  • Codon
  • DNA, Neoplasm/analysis
  • Female
  • Flow Cytometry
  • Genes, ras
  • Humans
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Point Mutation
  • Polymorphism, Restriction Fragment Length
  • Stomach Neoplasms/genetics

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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