Clinical and microbiologic investigation of an expedited peri-implantitis dog model: An animal study

Wook Jin Seong, Georgios Kotsakis, Jong Ki Huh, Soo Cheol Jeong, Ki Young Nam, Jong Ryul Kim, Young Cheul Heo, Hyeon Cheol Kim, Lei Zhang, Michael D. Evans, Heather Conrad, Robert J. Schumacher

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Background: Animal studies are pivotal in allowing experimentation to identify efficacious treatment protocols for resolution of peri-implantitis. The purpose of this investigation was to characterize an expedited dog peri-implantitis model clinically, radiographically, and microbiologically. Methods: Eight hound dogs underwent extractions (week 0) and implant (3.3 × 8.5 mm) placement with simultaneous surgical defect creation and ligature placement for induction of peri-implantitis (week 10). Ligatures were replaced at 6 weeks (week 16) and removed after 9 weeks (week 19) when supporting bone loss involved approximately 50% of the peri-implant bone. Microbial samples from the defects and healthy control implant sites collected at week 19 were analyzed utilizing a microarray. Clinical measures of inflammation were obtained and radiographic bone loss was measured from periapical radiographs. Radiographic depth and width measurements of bony defect were repeated at weeks 10 (baseline), 16, and 19. Canonical analysis of principal coordinates was used to visualize overall differences in microbial abundance between peri-implantitis and healthy implants. Results: This accelerated disease protocol led to intrabony defect creation with a mean depth and width of 4.3 mm and 3.5 mm, respectively after 9 weeks of ligature placement. Microbial identification revealed 59 total bacteria in peri-implant sites, 21 of which were only present in peri-implant sites as compared to healthy controls. Overall microbial beta diversity (microbial between-sample compositional diversity) differed between peri-implantitis and healthy implants (p = 0.009). Conclusions: Within the limitations of this study, this protocol led to expedited generation of peri-implant defects with a microbial profile indicative of a shift to disease and defect patterns conducive to regenerative treatment. However, the possibility of potential spontaneous resolution of lesions due to the lack of a chronicity interval as compared to chronic disease models need to be further clarified and considered during preclinical peri-implantitis model selection.

Original languageEnglish (US)
Article number150
JournalBMC Oral Health
Volume19
Issue number1
DOIs
StatePublished - Jul 15 2019

Bibliographical note

Funding Information:
This animal study was funded by the Center for Translational Medicine at the University of Minnesota. Research reported in this publication was supported by the National Center for Advancing Translational Sciences of the National Institutes of Health awards UL1TR000114 and UL1TR002494. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Publisher Copyright:
© 2019 The Author(s).

Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.

Keywords

  • Dental implant
  • Expedited dog model
  • Peri-implantitis

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