Class I-like HLA genes map telomeric to the HLA-A2 locus in human cells

Harry T. Orr, Robert Demars

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Among the proteins encoded by the major histocompatibility complex (MHC) are the highly polymorphic class I major transplantation antigens, HLA-A and HLA-B in man and H-2K and H-2D in mouse. Class I loci also include the less polymorphic HLA-C in man and H-2L in mouse. Class I antigens are 45,000-molecular weight (Mr) glycosylated membrane proteins associated on the cell surface with apparently nonpolymorphic β2-microglobulin1. Linked to the murine MHC are genes encoding the class I-like Qa and Tla antigens, which are closely related structurally to H-2K, D and L, as they are also 45,000-Mr cell surface glycoproteins associated with β2-microglobulin 2-7. Such class I molecules have also been found on the surface of human T lymphocytes8-11 and, in one case, shown to be linked to HLA-A12. Recombinant DNA techniques have been used to map DNA fragments to the Tla region in mouse13,14. A similar genetic analysis of the HLA complex is hampered by the lack of congeneic strains and by the small number of intra-MHC recombinants in well studied families. One means of overcoming these problems involves a molecular genetic analysis of human lymphoblastoid cell line (LCL) mutants15 having γ-ray-induced physical deletions of HLA DNA to map DNA fragments in the MHC16. Here we have applied this method to provide evidence that some human class I-like DNA sequences are telomeric to the A2 locus in LCL 721.

Original languageEnglish (US)
Pages (from-to)534-536
Number of pages3
JournalNature
Volume302
Issue number5908
DOIs
StatePublished - Dec 1 1983

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MHC Class I Genes
HLA-A2 Antigen
Major Histocompatibility Complex
DNA
HLA-C Antigens
Cell Line
Histocompatibility Antigens Class I
Histocompatibility Antigens
compound A 12
HLA-A Antigens
HLA-B Antigens
Recombinant DNA
Membrane Glycoproteins
varespladib methyl
Molecular Biology
Membrane Proteins
Molecular Weight
Proteins

Cite this

Class I-like HLA genes map telomeric to the HLA-A2 locus in human cells. / Orr, Harry T.; Demars, Robert.

In: Nature, Vol. 302, No. 5908, 01.12.1983, p. 534-536.

Research output: Contribution to journalArticle

Orr, Harry T. ; Demars, Robert. / Class I-like HLA genes map telomeric to the HLA-A2 locus in human cells. In: Nature. 1983 ; Vol. 302, No. 5908. pp. 534-536.
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