Abstract

Objective: We characterized the state of the vascular endothelium in pediatric obesity by comparing circulating endothelial cell (CEC) number and activation phenotype in severely obese children to that of normal weight, overweight, and obese children. Study design: We used immunohistochemical examination of buffy-coat smears to enumerate CEC and immunofluorescence microscopy to quantify activated CEC in 107 children and adolescents. Normal weight (body mass index [BMI] <85th percentile; n = 40), overweight (BMI 85th-<95th percentile; n = 17), and obese (BMI 95th-<99th percentile; n = 23) participants were recruited from a longitudinal study. Severely obese (BMI ≥99th percentile; n = 27) participants were recruited from a pediatric obesity clinic. Group means (adiposity; systolic blood pressure [SBP] quartiles) were compared with general linear models, adjusted for sex, age, and race. With Pearson correlations, we characterized relations of CEC with cardiovascular risk factors. Results: Activated CEC increased across BMI groups (P < .002) and SBP quartiles (P < .05). CEC number and activated CEC were highest in the severely obese group. CEC number was significantly associated with SBP, diastolic blood pressure, and triglycerides level. Activated CEC were significantly associated with SBP and high-density lipoprotein cholesterol levels. Conclusions: The vascular endothelium was activated in relation to excess adiposity, particularly in severely obese children, and to elevated SBP in children and adolescents.

Original languageEnglish (US)
Pages (from-to)547-551
Number of pages5
JournalJournal of Pediatrics
Volume157
Issue number4
DOIs
StatePublished - Oct 1 2010

Fingerprint

Pediatric Obesity
Endothelial Cells
Blood Pressure
Body Mass Index
Cell Count
Vascular Endothelium
Adiposity
Weights and Measures
Fluorescence Microscopy
HDL Cholesterol
Longitudinal Studies
Linear Models
Triglycerides

Cite this

@article{f4406ba53cd94883bced5ecaad05df4b,
title = "Circulating activated endothelial cells in pediatric obesity",
abstract = "Objective: We characterized the state of the vascular endothelium in pediatric obesity by comparing circulating endothelial cell (CEC) number and activation phenotype in severely obese children to that of normal weight, overweight, and obese children. Study design: We used immunohistochemical examination of buffy-coat smears to enumerate CEC and immunofluorescence microscopy to quantify activated CEC in 107 children and adolescents. Normal weight (body mass index [BMI] <85th percentile; n = 40), overweight (BMI 85th-<95th percentile; n = 17), and obese (BMI 95th-<99th percentile; n = 23) participants were recruited from a longitudinal study. Severely obese (BMI ≥99th percentile; n = 27) participants were recruited from a pediatric obesity clinic. Group means (adiposity; systolic blood pressure [SBP] quartiles) were compared with general linear models, adjusted for sex, age, and race. With Pearson correlations, we characterized relations of CEC with cardiovascular risk factors. Results: Activated CEC increased across BMI groups (P < .002) and SBP quartiles (P < .05). CEC number and activated CEC were highest in the severely obese group. CEC number was significantly associated with SBP, diastolic blood pressure, and triglycerides level. Activated CEC were significantly associated with SBP and high-density lipoprotein cholesterol levels. Conclusions: The vascular endothelium was activated in relation to excess adiposity, particularly in severely obese children, and to elevated SBP in children and adolescents.",
author = "Kelly, {Aaron S} and Hebbel, {Robert P} and Anna Solovey and Schwarzenberg, {Sarah J} and Metzig, {Andrea M.} and Antoinette Moran and Sinaiko, {Alan R} and {Jacobs Jr}, {David R} and Julia Steinberger",
year = "2010",
month = "10",
day = "1",
doi = "10.1016/j.jpeds.2010.04.069",
language = "English (US)",
volume = "157",
pages = "547--551",
journal = "Journal of Pediatrics",
issn = "0022-3476",
publisher = "Mosby Inc.",
number = "4",

}

TY - JOUR

T1 - Circulating activated endothelial cells in pediatric obesity

AU - Kelly, Aaron S

AU - Hebbel, Robert P

AU - Solovey, Anna

AU - Schwarzenberg, Sarah J

AU - Metzig, Andrea M.

AU - Moran, Antoinette

AU - Sinaiko, Alan R

AU - Jacobs Jr, David R

AU - Steinberger, Julia

PY - 2010/10/1

Y1 - 2010/10/1

N2 - Objective: We characterized the state of the vascular endothelium in pediatric obesity by comparing circulating endothelial cell (CEC) number and activation phenotype in severely obese children to that of normal weight, overweight, and obese children. Study design: We used immunohistochemical examination of buffy-coat smears to enumerate CEC and immunofluorescence microscopy to quantify activated CEC in 107 children and adolescents. Normal weight (body mass index [BMI] <85th percentile; n = 40), overweight (BMI 85th-<95th percentile; n = 17), and obese (BMI 95th-<99th percentile; n = 23) participants were recruited from a longitudinal study. Severely obese (BMI ≥99th percentile; n = 27) participants were recruited from a pediatric obesity clinic. Group means (adiposity; systolic blood pressure [SBP] quartiles) were compared with general linear models, adjusted for sex, age, and race. With Pearson correlations, we characterized relations of CEC with cardiovascular risk factors. Results: Activated CEC increased across BMI groups (P < .002) and SBP quartiles (P < .05). CEC number and activated CEC were highest in the severely obese group. CEC number was significantly associated with SBP, diastolic blood pressure, and triglycerides level. Activated CEC were significantly associated with SBP and high-density lipoprotein cholesterol levels. Conclusions: The vascular endothelium was activated in relation to excess adiposity, particularly in severely obese children, and to elevated SBP in children and adolescents.

AB - Objective: We characterized the state of the vascular endothelium in pediatric obesity by comparing circulating endothelial cell (CEC) number and activation phenotype in severely obese children to that of normal weight, overweight, and obese children. Study design: We used immunohistochemical examination of buffy-coat smears to enumerate CEC and immunofluorescence microscopy to quantify activated CEC in 107 children and adolescents. Normal weight (body mass index [BMI] <85th percentile; n = 40), overweight (BMI 85th-<95th percentile; n = 17), and obese (BMI 95th-<99th percentile; n = 23) participants were recruited from a longitudinal study. Severely obese (BMI ≥99th percentile; n = 27) participants were recruited from a pediatric obesity clinic. Group means (adiposity; systolic blood pressure [SBP] quartiles) were compared with general linear models, adjusted for sex, age, and race. With Pearson correlations, we characterized relations of CEC with cardiovascular risk factors. Results: Activated CEC increased across BMI groups (P < .002) and SBP quartiles (P < .05). CEC number and activated CEC were highest in the severely obese group. CEC number was significantly associated with SBP, diastolic blood pressure, and triglycerides level. Activated CEC were significantly associated with SBP and high-density lipoprotein cholesterol levels. Conclusions: The vascular endothelium was activated in relation to excess adiposity, particularly in severely obese children, and to elevated SBP in children and adolescents.

UR - http://www.scopus.com/inward/record.url?scp=77956392922&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956392922&partnerID=8YFLogxK

U2 - 10.1016/j.jpeds.2010.04.069

DO - 10.1016/j.jpeds.2010.04.069

M3 - Article

C2 - 20547395

AN - SCOPUS:77956392922

VL - 157

SP - 547

EP - 551

JO - Journal of Pediatrics

JF - Journal of Pediatrics

SN - 0022-3476

IS - 4

ER -