Circadian time structure of circulating plasma lipid peroxides, antioxidant enzymes and other small molecules in peptic ulcers

Ranjana Singh, Rajesh Kumar Singh, Tariq Masood, Anil Kumar Tripathi, Abbas Ali Mahdi, Raj Kumar Singh, Othild Schwartzkopff, Germaine Cornelissen

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Background: The circadian rhythm, as part of a broad time structure (chronome) of lipid peroxides and antioxidant defense mechanisms may relate to prevention, efficacy and management of preventive and curative chronotherapy. Methods: Fifty newly diagnosed patients with peptic ulcers, 30-45. years of age, and 60 age-matched clinically healthy volunteers were synchronized for one week with diurnal activity from about 06:00 to about 22:00 and nocturnal rest. Breakfast was served around 08:30, lunch around 13:30 and dinner around 20:30. Drugs known to affect the free-radical systems were not taken. Blood samples were collected at 6-hour intervals for 24. h under standardized, presumably 24-hour synchronized conditions. Plasma lipid peroxides, in the form of malondialdehyde (MDA), blood superoxide dismutase (SOD), glutathione peroxide (GPx), glutathione reductase (GR), catalase (CAT) activities, and serum total protein, albumin, ascorbic acid, total serum cholesterol, and HDL-cholesterol concentrations were determined. Results: By population-mean cosinor analysis, a marked circadian variation was demonstrated for all variables in healthy subjects and in ulcer patients (p. <. 0.001). As compared to controls, patients had a lower MESOR of MDA, SOD, GPx, GR, ascorbic acid, and HDL-C. They also had smaller circadian amplitude of SOD, CAT, GPx, GR, ascorbic acid, T-C, and HDL-C, but larger circadian amplitude of MDA and albumin. As compared to healthy subjects, the circadian acrophase of ulcer patients occurred later for MDA and GR and earlier for GPx. Conclusion: Mapping circadian rhythms, important chronome components that include trends with age and extra-circadian components characterizing antioxidants and pro-oxidants, is needed for exploring their putative role as markers in the treatment and management of peptic ulcers.

Original languageEnglish (US)
Pages (from-to)222-226
Number of pages5
JournalClinica Chimica Acta
StatePublished - Dec 7 2015

Bibliographical note

Publisher Copyright:
© 2015.


  • Antioxidant defense mechanisms
  • Chronome
  • Chronoprevention
  • Circadian
  • Gastric and/or duodenal mucosal erosion and ulceration
  • Lipid peroxides
  • Marker rhythm


Dive into the research topics of 'Circadian time structure of circulating plasma lipid peroxides, antioxidant enzymes and other small molecules in peptic ulcers'. Together they form a unique fingerprint.

Cite this