Circadian telomerase activity and DNA synthesis for timing peptide administration

Yi Qu, Zhengrong Wang, Xiang Huang, Chaomin Wan, Chun Lei Yang, Bailin Liu, Germaine G Cornelissen-Guillaume, Franz Halberg

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

DNA synthesis and telomerase activity were assessed in nude mice transplanted with hepatic carcinoma. Hepatic cancer cells (SMMC-7721) were implanted into both flanks of each of 14 BALB/C mice synchronized in 12h of light alternating with 12h of darkness (LD12:12) for 4 weeks. At 7 timepoints, tumor samples were collected for measurement of cellular DNA content by flow cytometry and telomerase activity by PCR-ELISA assay. Cosinor analyses determine a 24-h rhythm for all variables, showing a similar timing for the DNA-synthesis phase and telomerase activity. These results provide a model for exploring optimal timing of chronotherapy with peptides, especially for treatment with telomerase inhibitors.

Original languageEnglish (US)
Pages (from-to)363-369
Number of pages7
JournalPeptides
Volume24
Issue number3
DOIs
StatePublished - Mar 1 2003

Bibliographical note

Funding Information:
National Nature Science Foundation of China (project 39970275, 30070198 for W.Z. and W.C.); U.S. Public Health Service (GM-13981) (F.H.); Dr. h.c. Earl Bakken Fund (G.C., F.H.); University of Minnesota Supercomputing Institute (G.C., F.H.).

Keywords

  • Cell cycle
  • Circadian rhythm
  • DNA synthesis
  • Hepatic carcinoma
  • Nude mice
  • Telomerase activity

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