Circadian telomerase activity and DNA synthesis for timing peptide administration

Yi Qu; Zhengrong Wang; Xiang Huang; Chaomin Wan; Chun Lei Yang; Bailin Liu; Germaine G Cornelissen-Guillaume; Franz Halberg

doi:10.1016/S0196-9781(03)00050-0

Circadian telomerase activity and DNA synthesis for timing peptide administration

Yi Qu, Zhengrong Wang, Xiang Huang, Chaomin Wan, Chun Lei Yang, Bailin Liu, Germaine G Cornelissen-Guillaume, Franz Halberg

Physiology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

DNA synthesis and telomerase activity were assessed in nude mice transplanted with hepatic carcinoma. Hepatic cancer cells (SMMC-7721) were implanted into both flanks of each of 14 BALB/C mice synchronized in 12h of light alternating with 12h of darkness (LD12:12) for 4 weeks. At 7 timepoints, tumor samples were collected for measurement of cellular DNA content by flow cytometry and telomerase activity by PCR-ELISA assay. Cosinor analyses determine a 24-h rhythm for all variables, showing a similar timing for the DNA-synthesis phase and telomerase activity. These results provide a model for exploring optimal timing of chronotherapy with peptides, especially for treatment with telomerase inhibitors.

Original language	English (US)
Pages (from-to)	363-369
Number of pages	7
Journal	Peptides
Volume	24
Issue number	3
DOIs	https://doi.org/10.1016/S0196-9781(03)00050-0
State	Published - Mar 1 2003

Bibliographical note

Funding Information:
National Nature Science Foundation of China (project 39970275, 30070198 for W.Z. and W.C.); U.S. Public Health Service (GM-13981) (F.H.); Dr. h.c. Earl Bakken Fund (G.C., F.H.); University of Minnesota Supercomputing Institute (G.C., F.H.).

Keywords

Cell cycle
Circadian rhythm
DNA synthesis
Hepatic carcinoma
Nude mice
Telomerase activity

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title = "Circadian telomerase activity and DNA synthesis for timing peptide administration",

abstract = "DNA synthesis and telomerase activity were assessed in nude mice transplanted with hepatic carcinoma. Hepatic cancer cells (SMMC-7721) were implanted into both flanks of each of 14 BALB/C mice synchronized in 12h of light alternating with 12h of darkness (LD12:12) for 4 weeks. At 7 timepoints, tumor samples were collected for measurement of cellular DNA content by flow cytometry and telomerase activity by PCR-ELISA assay. Cosinor analyses determine a 24-h rhythm for all variables, showing a similar timing for the DNA-synthesis phase and telomerase activity. These results provide a model for exploring optimal timing of chronotherapy with peptides, especially for treatment with telomerase inhibitors.",

keywords = "Cell cycle, Circadian rhythm, DNA synthesis, Hepatic carcinoma, Nude mice, Telomerase activity",

author = "Yi Qu and Zhengrong Wang and Xiang Huang and Chaomin Wan and Yang, {Chun Lei} and Bailin Liu and Cornelissen-Guillaume, {Germaine G} and Franz Halberg",

note = "Funding Information: National Nature Science Foundation of China (project 39970275, 30070198 for W.Z. and W.C.); U.S. Public Health Service (GM-13981) (F.H.); Dr. h.c. Earl Bakken Fund (G.C., F.H.); University of Minnesota Supercomputing Institute (G.C., F.H.). ",

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AU - Qu, Yi

AU - Wang, Zhengrong

AU - Huang, Xiang

AU - Wan, Chaomin

AU - Yang, Chun Lei

AU - Liu, Bailin

AU - Cornelissen-Guillaume, Germaine G

AU - Halberg, Franz

N1 - Funding Information: National Nature Science Foundation of China (project 39970275, 30070198 for W.Z. and W.C.); U.S. Public Health Service (GM-13981) (F.H.); Dr. h.c. Earl Bakken Fund (G.C., F.H.); University of Minnesota Supercomputing Institute (G.C., F.H.).

PY - 2003/3/1

Y1 - 2003/3/1

N2 - DNA synthesis and telomerase activity were assessed in nude mice transplanted with hepatic carcinoma. Hepatic cancer cells (SMMC-7721) were implanted into both flanks of each of 14 BALB/C mice synchronized in 12h of light alternating with 12h of darkness (LD12:12) for 4 weeks. At 7 timepoints, tumor samples were collected for measurement of cellular DNA content by flow cytometry and telomerase activity by PCR-ELISA assay. Cosinor analyses determine a 24-h rhythm for all variables, showing a similar timing for the DNA-synthesis phase and telomerase activity. These results provide a model for exploring optimal timing of chronotherapy with peptides, especially for treatment with telomerase inhibitors.

AB - DNA synthesis and telomerase activity were assessed in nude mice transplanted with hepatic carcinoma. Hepatic cancer cells (SMMC-7721) were implanted into both flanks of each of 14 BALB/C mice synchronized in 12h of light alternating with 12h of darkness (LD12:12) for 4 weeks. At 7 timepoints, tumor samples were collected for measurement of cellular DNA content by flow cytometry and telomerase activity by PCR-ELISA assay. Cosinor analyses determine a 24-h rhythm for all variables, showing a similar timing for the DNA-synthesis phase and telomerase activity. These results provide a model for exploring optimal timing of chronotherapy with peptides, especially for treatment with telomerase inhibitors.

KW - Cell cycle

KW - Circadian rhythm

KW - DNA synthesis

KW - Hepatic carcinoma

KW - Nude mice

KW - Telomerase activity

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