Circadian telomerase activity and DNA synthesis for timing peptide administration

Yi Qu; Zhengrong Wang; Xiang Huang; Chaomin Wan; Chun Lei Yang; Bailin Liu; Germaine G Cornelissen-Guillaume; Franz Halberg

doi:10.1016/S0196-9781(03)00050-0

Circadian telomerase activity and DNA synthesis for timing peptide administration

Yi Qu, Zhengrong Wang, Xiang Huang, Chaomin Wan, Chun Lei Yang, Bailin Liu, Germaine G Cornelissen-Guillaume, Franz Halberg

Physiology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

DNA synthesis and telomerase activity were assessed in nude mice transplanted with hepatic carcinoma. Hepatic cancer cells (SMMC-7721) were implanted into both flanks of each of 14 BALB/C mice synchronized in 12h of light alternating with 12h of darkness (LD12:12) for 4 weeks. At 7 timepoints, tumor samples were collected for measurement of cellular DNA content by flow cytometry and telomerase activity by PCR-ELISA assay. Cosinor analyses determine a 24-h rhythm for all variables, showing a similar timing for the DNA-synthesis phase and telomerase activity. These results provide a model for exploring optimal timing of chronotherapy with peptides, especially for treatment with telomerase inhibitors.

Original language	English (US)
Pages (from-to)	363-369
Number of pages	7
Journal	Peptides
Volume	24
Issue number	3
DOIs	https://doi.org/10.1016/S0196-9781(03)00050-0
State	Published - Mar 1 2003

Bibliographical note

Funding Information:
National Nature Science Foundation of China (project 39970275, 30070198 for W.Z. and W.C.); U.S. Public Health Service (GM-13981) (F.H.); Dr. h.c. Earl Bakken Fund (G.C., F.H.); University of Minnesota Supercomputing Institute (G.C., F.H.).

Keywords

Cell cycle
Circadian rhythm
DNA synthesis
Hepatic carcinoma
Nude mice
Telomerase activity

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0196-9781(03)00050-0

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title = "Circadian telomerase activity and DNA synthesis for timing peptide administration",

abstract = "DNA synthesis and telomerase activity were assessed in nude mice transplanted with hepatic carcinoma. Hepatic cancer cells (SMMC-7721) were implanted into both flanks of each of 14 BALB/C mice synchronized in 12h of light alternating with 12h of darkness (LD12:12) for 4 weeks. At 7 timepoints, tumor samples were collected for measurement of cellular DNA content by flow cytometry and telomerase activity by PCR-ELISA assay. Cosinor analyses determine a 24-h rhythm for all variables, showing a similar timing for the DNA-synthesis phase and telomerase activity. These results provide a model for exploring optimal timing of chronotherapy with peptides, especially for treatment with telomerase inhibitors.",

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note = "Funding Information: National Nature Science Foundation of China (project 39970275, 30070198 for W.Z. and W.C.); U.S. Public Health Service (GM-13981) (F.H.); Dr. h.c. Earl Bakken Fund (G.C., F.H.); University of Minnesota Supercomputing Institute (G.C., F.H.).",

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AU - Qu, Yi

AU - Wang, Zhengrong

AU - Huang, Xiang

AU - Wan, Chaomin

AU - Yang, Chun Lei

AU - Liu, Bailin

AU - Cornelissen-Guillaume, Germaine G

AU - Halberg, Franz

N1 - Funding Information: National Nature Science Foundation of China (project 39970275, 30070198 for W.Z. and W.C.); U.S. Public Health Service (GM-13981) (F.H.); Dr. h.c. Earl Bakken Fund (G.C., F.H.); University of Minnesota Supercomputing Institute (G.C., F.H.).

PY - 2003/3/1

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N2 - DNA synthesis and telomerase activity were assessed in nude mice transplanted with hepatic carcinoma. Hepatic cancer cells (SMMC-7721) were implanted into both flanks of each of 14 BALB/C mice synchronized in 12h of light alternating with 12h of darkness (LD12:12) for 4 weeks. At 7 timepoints, tumor samples were collected for measurement of cellular DNA content by flow cytometry and telomerase activity by PCR-ELISA assay. Cosinor analyses determine a 24-h rhythm for all variables, showing a similar timing for the DNA-synthesis phase and telomerase activity. These results provide a model for exploring optimal timing of chronotherapy with peptides, especially for treatment with telomerase inhibitors.

AB - DNA synthesis and telomerase activity were assessed in nude mice transplanted with hepatic carcinoma. Hepatic cancer cells (SMMC-7721) were implanted into both flanks of each of 14 BALB/C mice synchronized in 12h of light alternating with 12h of darkness (LD12:12) for 4 weeks. At 7 timepoints, tumor samples were collected for measurement of cellular DNA content by flow cytometry and telomerase activity by PCR-ELISA assay. Cosinor analyses determine a 24-h rhythm for all variables, showing a similar timing for the DNA-synthesis phase and telomerase activity. These results provide a model for exploring optimal timing of chronotherapy with peptides, especially for treatment with telomerase inhibitors.

KW - Cell cycle

KW - Circadian rhythm

KW - DNA synthesis

KW - Hepatic carcinoma

KW - Nude mice

KW - Telomerase activity

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Circadian telomerase activity and DNA synthesis for timing peptide administration

Abstract

Bibliographical note

Keywords

UN SDGs

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