Circadian renal rhythms influenced by implanted encapsulated hANP-producing cells in Goldblatt hypertensive rats

L. G. Chen, Z. R. Wang, C. M. Wan, J. Xiao, L. Guo, H. L. Guo, Germaine G Cornelissen-Guillaume, F. Halberg

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Renal excretion in experimental hypertensive rats implanted with encapsulated human atrial natriuretic peptide (hANP)-producing cells is circadian periodic. Chinese hamster ovary (CHO) cells transfected with the plasmid hANP-cDNA were encapsulated in biocompatible polycaprolactone capsules for intraperitoneal implantation into two-kidney, one-clip (2K1C) hypertensive rats. During a 12:12 light-dark cycle, as compared to control CHO cells, the implantation of encapsulated hANP-producing CHO cells was associated with an increase in the net excretion of water, sodium and potassium, and with a reversal of the advanced circadian phases related to renovascular hypertension in 2K1C rats. The increase in blood pressure postimplantation was delayed, and increases in renal blood flow, glomerular filtration rate, sodium output, urinary excretion and urinary cyclic GMP concentrations were also found. Implantation of encapsulated hANP-producing cells affects circadian rhythms in kidney excretion functions of 2K1C rats, and may be useful for the treatment of cardiovascular disease.

Original languageEnglish (US)
Pages (from-to)1515-1522
Number of pages8
JournalGene therapy
Issue number20
StatePublished - Oct 2004

Bibliographical note

Funding Information:
This study was supported by the National Nature Science Foundation of China (Nos. 39970275, 30070278 to Wang Z; No. 30070198 to Wan C), by the US Public Health Service (GM–13981) (FH), and the Dr h.c. Dr h.c. Earl Bakken Fund (FH, GC).


  • Atrial natriuretic peptide
  • Circadian rhythm
  • Encapsulated transfected cells
  • Hypertension


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