Ciliary and flagellar motility and the nexin-dynein regulatory complex

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Nexin was first identified in electron microscope images of axonemes as a linker that repeats every 96. nm between adjacent outer doublet microtubules. The nexin links are thought to be protease-sensitive structures that limit microtubule sliding during the ciliary beat cycle. The dynein regulatory complex (DRC) was first described in genetic screens for suppressor mutations that restore motility to paralyzed radial spoke (RS) or central pair (CP) mutants. The DRC is thought to identify intermediates in the signaling pathway between the CP, RS, and dynein arms. Cryo-electron tomography of drc mutant axonemes revealed that the DRC is located within the nexin link, in close proximity to RS2 and a calmodulin spoke complex, and also serves as an adaptor that stabilizes the assembly of several inner arm dyneins. Recent studies have identified at least 11 DRC subunits and several other N-DRC-associated proteins. The implications of these findings for future studies of ciliary motility are discussed.

Original languageEnglish (US)
Title of host publicationDyneins
Subtitle of host publicationThe Biology of Dynein Motors: Second Edition
PublisherElsevier Inc.
Pages299-335
Number of pages37
ISBN (Electronic)9780128097021
ISBN (Print)9780128094716
DOIs
StatePublished - Nov 22 2017

    Fingerprint

Keywords

  • Cilia
  • Dynein regulatory complex
  • Flagella
  • Interdoublet links
  • Nexin
  • Radial spokes

Cite this

Porter, M. E. (2017). Ciliary and flagellar motility and the nexin-dynein regulatory complex. In Dyneins: The Biology of Dynein Motors: Second Edition (pp. 299-335). Elsevier Inc.. https://doi.org/10.1016/B978-0-12-809471-6.00010-3