Chronomics of Circulating Plasma Lipid Peroxides and Antioxidant Enzymes in Renal Stone Formers

Rajeev Singh Kushwaha, R. C. Gupta, Sumita Sharma, Tariq Masood, J. P. Sharma, Rajesh K. Singh, Raj K. Singh, Cathy L. Gierke, Germaine G Cornelissen-Guillaume

Research output: Contribution to journalArticle

Abstract

The chronome of lipid peroxidation and anti-oxidant defense mechanisms may relate to the efficacy and management of time qualified preventive therapeutic and dietary interventions. One hundred renal stone patients, 20–60 years of age, and 50 clinically healthy volunteers, 21–45 years, were synchronized for 1 week with diurnal activity from 06:00 to 22:00 and nocturnal rest. All subjects took their usual meals three times daily (breakfast around 08:30, lunch around 13:00, and dinner around 20:30) with usual fluid intake. Drugs known to affect free radical system were not taken. Blood samples were collected at 6-h intervals for 24-h under standardized, presumably 24-h synchronized conditions. Determinations included plasma lipid peroxides, in terms of malondialdehyde (MDA) and blood superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR) and catalase (CAT) activities. A marked circadian variation was demonstrated for each studied variable by population-mean cosinor in renal stone patients and healthy participants (p < 0.001). By comparison to healthy subjects, parameter tests indicate that the stone formers had a higher MESOR of MDA, but a lower MESOR of SOD, GPx, GR and CAT. Furthermore, the patients also differed from the healthy controls in terms of their circadian amplitude and acrophase (tested jointly) of all variables (p < 0.001). Mapping the broader time structure with multifrequency circadian characteristics of oxidants and anti-oxidants is needed for exploring their role as marker in the treatment and management of urolithiasis.

Original languageEnglish (US)
Pages (from-to)195-200
Number of pages6
JournalIndian Journal of Clinical Biochemistry
Volume34
Issue number2
DOIs
StatePublished - Apr 1 2019

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Lipid Peroxides
Oxidants
Healthy Volunteers
Glutathione Reductase
Antioxidants
Glutathione Peroxidase
Malondialdehyde
Kidney
Plasmas
Catalase
Superoxide Dismutase
Meals
Blood
Enzymes
Time Management
Lunch
Urolithiasis
Breakfast
Defense Mechanisms
Lipid Peroxidation

Keywords

  • CAT
  • Chronomics
  • GPx
  • GR
  • Lipid peroxides
  • MDA
  • Renal stone formers
  • SOD
  • Urolithiasis

Cite this

Chronomics of Circulating Plasma Lipid Peroxides and Antioxidant Enzymes in Renal Stone Formers. / Kushwaha, Rajeev Singh; Gupta, R. C.; Sharma, Sumita; Masood, Tariq; Sharma, J. P.; Singh, Rajesh K.; Singh, Raj K.; Gierke, Cathy L.; Cornelissen-Guillaume, Germaine G.

In: Indian Journal of Clinical Biochemistry, Vol. 34, No. 2, 01.04.2019, p. 195-200.

Research output: Contribution to journalArticle

Kushwaha, Rajeev Singh ; Gupta, R. C. ; Sharma, Sumita ; Masood, Tariq ; Sharma, J. P. ; Singh, Rajesh K. ; Singh, Raj K. ; Gierke, Cathy L. ; Cornelissen-Guillaume, Germaine G. / Chronomics of Circulating Plasma Lipid Peroxides and Antioxidant Enzymes in Renal Stone Formers. In: Indian Journal of Clinical Biochemistry. 2019 ; Vol. 34, No. 2. pp. 195-200.
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abstract = "The chronome of lipid peroxidation and anti-oxidant defense mechanisms may relate to the efficacy and management of time qualified preventive therapeutic and dietary interventions. One hundred renal stone patients, 20–60 years of age, and 50 clinically healthy volunteers, 21–45 years, were synchronized for 1 week with diurnal activity from 06:00 to 22:00 and nocturnal rest. All subjects took their usual meals three times daily (breakfast around 08:30, lunch around 13:00, and dinner around 20:30) with usual fluid intake. Drugs known to affect free radical system were not taken. Blood samples were collected at 6-h intervals for 24-h under standardized, presumably 24-h synchronized conditions. Determinations included plasma lipid peroxides, in terms of malondialdehyde (MDA) and blood superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR) and catalase (CAT) activities. A marked circadian variation was demonstrated for each studied variable by population-mean cosinor in renal stone patients and healthy participants (p < 0.001). By comparison to healthy subjects, parameter tests indicate that the stone formers had a higher MESOR of MDA, but a lower MESOR of SOD, GPx, GR and CAT. Furthermore, the patients also differed from the healthy controls in terms of their circadian amplitude and acrophase (tested jointly) of all variables (p < 0.001). Mapping the broader time structure with multifrequency circadian characteristics of oxidants and anti-oxidants is needed for exploring their role as marker in the treatment and management of urolithiasis.",
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