TY - JOUR
T1 - Chronomics of Circulating Plasma Lipid Peroxides and Antioxidant Enzymes in Renal Stone Formers
AU - Kushwaha, Rajeev Singh
AU - Gupta, R. C.
AU - Sharma, Sumita
AU - Masood, Tariq
AU - Sharma, J. P.
AU - Singh, Rajesh K.
AU - Singh, Raj K.
AU - Gierke, Cathy L.
AU - Cornelissen, Germaine
N1 - Publisher Copyright:
© 2017, Association of Clinical Biochemists of India.
PY - 2019/4/1
Y1 - 2019/4/1
N2 - The chronome of lipid peroxidation and anti-oxidant defense mechanisms may relate to the efficacy and management of time qualified preventive therapeutic and dietary interventions. One hundred renal stone patients, 20–60 years of age, and 50 clinically healthy volunteers, 21–45 years, were synchronized for 1 week with diurnal activity from 06:00 to 22:00 and nocturnal rest. All subjects took their usual meals three times daily (breakfast around 08:30, lunch around 13:00, and dinner around 20:30) with usual fluid intake. Drugs known to affect free radical system were not taken. Blood samples were collected at 6-h intervals for 24-h under standardized, presumably 24-h synchronized conditions. Determinations included plasma lipid peroxides, in terms of malondialdehyde (MDA) and blood superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR) and catalase (CAT) activities. A marked circadian variation was demonstrated for each studied variable by population-mean cosinor in renal stone patients and healthy participants (p < 0.001). By comparison to healthy subjects, parameter tests indicate that the stone formers had a higher MESOR of MDA, but a lower MESOR of SOD, GPx, GR and CAT. Furthermore, the patients also differed from the healthy controls in terms of their circadian amplitude and acrophase (tested jointly) of all variables (p < 0.001). Mapping the broader time structure with multifrequency circadian characteristics of oxidants and anti-oxidants is needed for exploring their role as marker in the treatment and management of urolithiasis.
AB - The chronome of lipid peroxidation and anti-oxidant defense mechanisms may relate to the efficacy and management of time qualified preventive therapeutic and dietary interventions. One hundred renal stone patients, 20–60 years of age, and 50 clinically healthy volunteers, 21–45 years, were synchronized for 1 week with diurnal activity from 06:00 to 22:00 and nocturnal rest. All subjects took their usual meals three times daily (breakfast around 08:30, lunch around 13:00, and dinner around 20:30) with usual fluid intake. Drugs known to affect free radical system were not taken. Blood samples were collected at 6-h intervals for 24-h under standardized, presumably 24-h synchronized conditions. Determinations included plasma lipid peroxides, in terms of malondialdehyde (MDA) and blood superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR) and catalase (CAT) activities. A marked circadian variation was demonstrated for each studied variable by population-mean cosinor in renal stone patients and healthy participants (p < 0.001). By comparison to healthy subjects, parameter tests indicate that the stone formers had a higher MESOR of MDA, but a lower MESOR of SOD, GPx, GR and CAT. Furthermore, the patients also differed from the healthy controls in terms of their circadian amplitude and acrophase (tested jointly) of all variables (p < 0.001). Mapping the broader time structure with multifrequency circadian characteristics of oxidants and anti-oxidants is needed for exploring their role as marker in the treatment and management of urolithiasis.
KW - CAT
KW - Chronomics
KW - GPx
KW - GR
KW - Lipid peroxides
KW - MDA
KW - Renal stone formers
KW - SOD
KW - Urolithiasis
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U2 - 10.1007/s12291-017-0726-6
DO - 10.1007/s12291-017-0726-6
M3 - Article
C2 - 31092993
AN - SCOPUS:85038613468
SN - 0970-1915
VL - 34
SP - 195
EP - 200
JO - Indian Journal of Clinical Biochemistry
JF - Indian Journal of Clinical Biochemistry
IS - 2
ER -