Chronobiologic approach to beat-to-beat variations of cultured murine myocardial cells

Hüiwan Han, Dali Shao, Jinyi Wu, Germaine G Cornelissen-Guillaume, Franz Halberg

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12 Scopus citations


An earlier demonstration of a circadian rhythm in rat atria by others is complemented herein by observations in culture: A single murine myocardial cell and two sets of grouped cells beating in culture for several days reveal several features of an anticipated, presumably built-in spectrum of multifrequency rhythms and trends, the chronome. Circadian and about 12-h (circasemidian) components are modulated by an approximately 84-h (circasemiseptan) component, which cannot be separated from trends in view of the brevity of the series. The circumstance under which the culture is aging and in which fibroblasts proliferate is a further complication that limits the findings to a single cycle reproduced in three separate cultures. Whether it is a rhythm that repeats itself or a response to placement into culture, an approximately 3.5-d component in the beating of myocardial cells in culture is to be aligned with a very prominent similar component found in the incidence of 85,819 human myocardial infarctions.

Original languageEnglish (US)
Pages (from-to)217-229
Number of pages13
JournalCell Biophysics
Issue number3
StatePublished - Jun 1991


  • Circadian
  • caroliac myocyte
  • chronobiology
  • circasemidian
  • circasemiseptan, chronome


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