Chromosome Congression by Kinesin-5 Motor-Mediated Disassembly of Longer Kinetochore Microtubules

Melissa K Gardner, David C. Bouck, Leocadia V. Paliulis, Janet B. Meehl, Eileen T. O'Toole, Julian Haase, Adelheid Soubry, Ajit P. Joglekar, Mark Winey, Edward D. Salmon, Kerry Bloom, David J Odde

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

During mitosis, sister chromatids congress to the spindle equator and are subsequently segregated via attachment to dynamic kinetochore microtubule (kMT) plus ends. A major question is how kMT plus-end assembly is spatially regulated to achieve chromosome congression. Here we find in budding yeast that the widely conserved kinesin-5 sliding motor proteins, Cin8p and Kip1p, mediate chromosome congression by suppressing kMT plus-end assembly of longer kMTs. Of the two, Cin8p is the major effector and its activity requires a functional motor domain. In contrast, the depolymerizing kinesin-8 motor Kip3p plays a minor role in spatial regulation of yeast kMT assembly. Our analysis identified a model where kinesin-5 motors bind to kMTs, move to kMT plus ends, and upon arrival at a growing plus end promote net kMT plus-end disassembly. In conclusion, we find that length-dependent control of net kMT assembly by kinesin-5 motors yields a simple and stable self-organizing mechanism for chromosome congression.

Original languageEnglish (US)
Pages (from-to)894-906
Number of pages13
JournalCell
Volume135
Issue number5
DOIs
StatePublished - Nov 28 2008

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Kinetochores
Kinesin
Chromosomes
Microtubules
Yeast
Saccharomycetales
Chromatids
Mitosis
Yeasts
Proteins

Keywords

  • MOLIMMUNO
  • SIGNALING

Cite this

Chromosome Congression by Kinesin-5 Motor-Mediated Disassembly of Longer Kinetochore Microtubules. / Gardner, Melissa K; Bouck, David C.; Paliulis, Leocadia V.; Meehl, Janet B.; O'Toole, Eileen T.; Haase, Julian; Soubry, Adelheid; Joglekar, Ajit P.; Winey, Mark; Salmon, Edward D.; Bloom, Kerry; Odde, David J.

In: Cell, Vol. 135, No. 5, 28.11.2008, p. 894-906.

Research output: Contribution to journalArticle

Gardner, MK, Bouck, DC, Paliulis, LV, Meehl, JB, O'Toole, ET, Haase, J, Soubry, A, Joglekar, AP, Winey, M, Salmon, ED, Bloom, K & Odde, DJ 2008, 'Chromosome Congression by Kinesin-5 Motor-Mediated Disassembly of Longer Kinetochore Microtubules', Cell, vol. 135, no. 5, pp. 894-906. https://doi.org/10.1016/j.cell.2008.09.046
Gardner, Melissa K ; Bouck, David C. ; Paliulis, Leocadia V. ; Meehl, Janet B. ; O'Toole, Eileen T. ; Haase, Julian ; Soubry, Adelheid ; Joglekar, Ajit P. ; Winey, Mark ; Salmon, Edward D. ; Bloom, Kerry ; Odde, David J. / Chromosome Congression by Kinesin-5 Motor-Mediated Disassembly of Longer Kinetochore Microtubules. In: Cell. 2008 ; Vol. 135, No. 5. pp. 894-906.
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