Chromosomal characteristics of ribosomal DNA in the primitive semionotiform fish, longnose gar Lepisosteus osseus

P. Ráb; M. Rábová; K. M. Reed; R. B. Phillips

doi:10.1023/A:1009202030456

Chromosomal characteristics of ribosomal DNA in the primitive semionotiform fish, longnose gar Lepisosteus osseus

P. Ráb, M. Rábová, K. M. Reed, R. B. Phillips

Genetics and Genomics

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The chromosomes of longnose gar, Lepisosteus osseus, an extant representative of early radiation of actinopterygian fishes, were studied using conventional Giemsa-staining, Ag-staining, CMA₃-fluorescence and fluorescence in-situ hybridization (FISH). The diploid chromosome number was 2n = 56 and the karyotype contained 11 pairs of metacentric, 6 pairs of submetacentric, 3 pairs of subtelocentric macrochromosomes and 16 microchromosomes. Nearly all macrochromosomes showed large CMA₃-positive regions resembling the R-bands of higher vertebrates, indicating extensive distribution of GC-rich DNA along chromosomes. The nucleolar organizer regions (NORs) were located on the end of the short arm of a single small metacentric macrochromosomal pair. These sites were strongly CMA₃-positive, suggesting that ribosomal sites are associated with GC-rich DNA. In-situ hybridization (FISH) with a rDNA probe gave consistently positive signals in the same regions detected by Ag-staining and CMA₃-fluorescence. The evolutionary conservation of positive CMA₃-fluorescence of ribosomal sites in 'holostean' and teleostean fishes is discussed.

Original language	English (US)
Pages (from-to)	475-480
Number of pages	6
Journal	Chromosome Research
Volume	7
Issue number	6
DOIs	https://doi.org/10.1023/A:1009202030456
State	Published - 1999

Bibliographical note

Funding Information:
This study was supported by grants from the Grant Agency of Academy of Sciences of CR A6045704 (P.R. and M.R.), US National Academy of Sciences (P.R. and R.B.P.), the NRI Competitive Grants Program/USDA (93-37205-9171) (K.M.R.). We greatly appreciate the technical assistance of Miss Jana BucÏková. A. Suzuki, Tokyo University of Agriculture, Hokkaido, kindly provided and translated the Japanese papre by Ueno (1985).

Keywords

Chromomycin A-banding
FISH
Fish cytogenetics
GC-rich heterochromatin
Longnose gar

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10.1023/A:1009202030456

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title = "Chromosomal characteristics of ribosomal DNA in the primitive semionotiform fish, longnose gar Lepisosteus osseus",

abstract = "The chromosomes of longnose gar, Lepisosteus osseus, an extant representative of early radiation of actinopterygian fishes, were studied using conventional Giemsa-staining, Ag-staining, CMA3-fluorescence and fluorescence in-situ hybridization (FISH). The diploid chromosome number was 2n = 56 and the karyotype contained 11 pairs of metacentric, 6 pairs of submetacentric, 3 pairs of subtelocentric macrochromosomes and 16 microchromosomes. Nearly all macrochromosomes showed large CMA3-positive regions resembling the R-bands of higher vertebrates, indicating extensive distribution of GC-rich DNA along chromosomes. The nucleolar organizer regions (NORs) were located on the end of the short arm of a single small metacentric macrochromosomal pair. These sites were strongly CMA3-positive, suggesting that ribosomal sites are associated with GC-rich DNA. In-situ hybridization (FISH) with a rDNA probe gave consistently positive signals in the same regions detected by Ag-staining and CMA3-fluorescence. The evolutionary conservation of positive CMA3-fluorescence of ribosomal sites in 'holostean' and teleostean fishes is discussed.",

keywords = "Chromomycin A-banding, FISH, Fish cytogenetics, GC-rich heterochromatin, Longnose gar",

author = "P. R{\'a}b and M. R{\'a}bov{\'a} and Reed, {K. M.} and Phillips, {R. B.}",

note = "Funding Information: This study was supported by grants from the Grant Agency of Academy of Sciences of CR A6045704 (P.R. and M.R.), US National Academy of Sciences (P.R. and R.B.P.), the NRI Competitive Grants Program/USDA (93-37205-9171) (K.M.R.). We greatly appreciate the technical assistance of Miss Jana Buc{\"I}kov{\'a}. A. Suzuki, Tokyo University of Agriculture, Hokkaido, kindly provided and translated the Japanese papre by Ueno (1985).",

year = "1999",

doi = "10.1023/A:1009202030456",

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AU - Ráb, P.

AU - Rábová, M.

AU - Reed, K. M.

AU - Phillips, R. B.

N1 - Funding Information: This study was supported by grants from the Grant Agency of Academy of Sciences of CR A6045704 (P.R. and M.R.), US National Academy of Sciences (P.R. and R.B.P.), the NRI Competitive Grants Program/USDA (93-37205-9171) (K.M.R.). We greatly appreciate the technical assistance of Miss Jana BucÏková. A. Suzuki, Tokyo University of Agriculture, Hokkaido, kindly provided and translated the Japanese papre by Ueno (1985).

PY - 1999

Y1 - 1999

N2 - The chromosomes of longnose gar, Lepisosteus osseus, an extant representative of early radiation of actinopterygian fishes, were studied using conventional Giemsa-staining, Ag-staining, CMA3-fluorescence and fluorescence in-situ hybridization (FISH). The diploid chromosome number was 2n = 56 and the karyotype contained 11 pairs of metacentric, 6 pairs of submetacentric, 3 pairs of subtelocentric macrochromosomes and 16 microchromosomes. Nearly all macrochromosomes showed large CMA3-positive regions resembling the R-bands of higher vertebrates, indicating extensive distribution of GC-rich DNA along chromosomes. The nucleolar organizer regions (NORs) were located on the end of the short arm of a single small metacentric macrochromosomal pair. These sites were strongly CMA3-positive, suggesting that ribosomal sites are associated with GC-rich DNA. In-situ hybridization (FISH) with a rDNA probe gave consistently positive signals in the same regions detected by Ag-staining and CMA3-fluorescence. The evolutionary conservation of positive CMA3-fluorescence of ribosomal sites in 'holostean' and teleostean fishes is discussed.

AB - The chromosomes of longnose gar, Lepisosteus osseus, an extant representative of early radiation of actinopterygian fishes, were studied using conventional Giemsa-staining, Ag-staining, CMA3-fluorescence and fluorescence in-situ hybridization (FISH). The diploid chromosome number was 2n = 56 and the karyotype contained 11 pairs of metacentric, 6 pairs of submetacentric, 3 pairs of subtelocentric macrochromosomes and 16 microchromosomes. Nearly all macrochromosomes showed large CMA3-positive regions resembling the R-bands of higher vertebrates, indicating extensive distribution of GC-rich DNA along chromosomes. The nucleolar organizer regions (NORs) were located on the end of the short arm of a single small metacentric macrochromosomal pair. These sites were strongly CMA3-positive, suggesting that ribosomal sites are associated with GC-rich DNA. In-situ hybridization (FISH) with a rDNA probe gave consistently positive signals in the same regions detected by Ag-staining and CMA3-fluorescence. The evolutionary conservation of positive CMA3-fluorescence of ribosomal sites in 'holostean' and teleostean fishes is discussed.

KW - Chromomycin A-banding

KW - FISH

KW - Fish cytogenetics

KW - GC-rich heterochromatin

KW - Longnose gar

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JF - Chromosome Research

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ER -

Chromosomal characteristics of ribosomal DNA in the primitive semionotiform fish, longnose gar Lepisosteus osseus

Abstract

Bibliographical note

Keywords

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